Research Progress of CRISPR/Cas9 System in Filamentous Fungi

doi:10.13560/j.cnki.biotech.bull.1985.2017-1128

Abstract

Abstract: Filamentous fungi,which are closely related to human survival,have been widely used in industrial development and application in addition to its great edible and medicinal values. In recent years,CRISPR/Cas9 has been rapidly developed in various fields due to its simple design,convenient operation,precise positioning and high efficiency,and has been received great attentions in the world. However,because of the relatively low level of homologous recombination in some fungi,such as Aspergillus fumigatus,genetic manipulation of them is rather difficult. Therefore,this technique is rarely used in filamentous fungi,especially in large fungi. Based on the latest application and research progress of CRISPR/Cas9 technology in filamentous fungi,this paper introduced various editing mechanisms in filamentous fungi,and analyzed the reported its application in model fungi,aiming at providing a reference to conduct gene editing for more fungi.

Key words: filamentous fungi, CRISPR/Cas9, gene editing, production applications

WANG Pei， LIU Wen， SHEN Xiang-ling. Research Progress of CRISPR/Cas9 System in Filamentous Fungi[J]. Biotechnology Bulletin, 2018, 34(4): 1-8.

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