Genetic Relationship Analysis of 22 Cordyceps militaris Based on Split Network and Preliminary Screening of High-yield Cordycepin Strains

doi:10.13560/j.cnki.biotech.bull.1985.2017-0852

Abstract

Abstract: We used the split network based on ITS sequence for analyzing the genetic relationships of 22 Cordyceps militaris strains,and we compared the capacities of strains producing cordycepin and screened the strains highly yielding cordycepin. The results showed that length of ITS sequence（including ITS1,5.8S rDNA and ITS 2）of 22 strains was 518-539 bp,there were 39 variable sites and 12 parsimony informative sites in the ITS sequence after alignment,and all occurred in the ITS1 region. The split network showed that 22 C. militaris strains were roughly divided into 3 clades,and the strains of each clade were distinguished from others by its unique base substitutions. Cordycepin was mainly in extracellular liquid,cordycepin contents by the 22 strains varied significantly,and the content of cordycepin in MF 27 strain was the highest at 332.74 mg/L,significantly higher than that by other strains（P<0.05）. This study provides a basis for understanding the systematic relationship among strains of C. militaris and the management of germplasm resources.

Key words: Cordyceps militaris, rDNA ITS sequences, split network, genetic relationship, variable sites, HPLC

LIU Jian-bing, QI Meng, DU Yuan-ru, FU Jun-sheng, HU Kai-hui. Genetic Relationship Analysis of 22 Cordyceps militaris Based on Split Network and Preliminary Screening of High-yield Cordycepin Strains[J]. Biotechnology Bulletin, 2018, 34(4): 133-138.

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