Genome-wide Identification and Expression Analysis of TPS Gene Family in Pear

doi:10.13560/j.cnki.biotech.bull.1985.2017-0804

Abstract

Abstract: Terpene synthases（TPS）are the key enzymes in the synthesis of terpenoids. The TPS gene family was identified and subfamilies were clustered based on the genome-wide database of Pyrus bretschneideri,then the structure of TPS genes,the characteristics of encoded proteins and expression patterns were analyzed by means of bioinformatics and molecular biology. The results showed that 33 TPS gene family members were identified and they were clustered into five subfamilies. Among them,TPS-a subfamily had the largest number of members and owned 4-7 exons;most of TPS proteins were located in cytoplasm,and a few of them were distributed in mitochondria,chloroplasts and endoplasmic reticulum. All pear TPS proteins were hydrophilic proteins,the secondary structure of most TPS proteins was α-helix and random coil,and the folded and extended strands scattered in it. TPS-a subfamily had the largest number of conserved motifs and less in TPS-e/f. Proteins in each subfamily were highly similar in tertiary structure. The qRT-PCR analysis of 10 TPS genes showed that 9 PbrTPS genes except PbrTPS24 expressed in roots,stems,young leaves and sprouts,each gene expressed highly in different part,indicating that the expression of PbrTPS gene was tissue-specific.

Key words: pear, terpene synthases, TPS gene family, bioinformatics, expression analysis

ZHAO Rong-rong, LIU Bin, GUO Chao, LIU Heng, ZHANG Yuan-hu. Genome-wide Identification and Expression Analysis of TPS Gene Family in Pear[J]. Biotechnology Bulletin, 2018, 34(5): 131-141.

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