Identification and Analysis of LBD Gene Family and Expression Analysis of Fruit Development in Cucumis melo

doi:10.13560/j.cnki.biotech.bull.1985.2022-1264

Abstract

Abstract:

The lateral organ boundaries domain（LBD）genes are widely found in higher plants, and can participate in the regulation of plant growth, development and stress response. Identification and analysis of the LBD transcription factor in melon（Cucumis melo）is aimed to unravel its regulatory role in the process of fruit development. Bioinformatics was used to identify the members of LBD transcription factor in melon genome and named as CmLBD1-CmLBD32. Multiple-sequence alignment was used to analyze the structure characteristics of LBD-domain in the melon. Maximum-likelihood and neighbor-joining were to have phylogenetic tree analyzed. The expressions of LBD gene family during fruit development were deteted by RT-qPCR using extracted RNAs from the fruits（five developing and mature fruits were harvested at 20, 25, 30, 35 and 40 d after pollination）. Total 32 LBD genes were identified from melon genome, and they can be divided into 7 categories and were distributed on 12 chromosomes. The multiple-sequence alignment analysis demonstrated that there were typical LOB domains in 32 LBD genes. LBD family genes expressed in different stages of fruits, the expressions of CmLBD2 and CmLBD28 gradually increased with the processes of fruit development, while relatively low for rest family members. Subcellular localization suggested that CmLBD2 protein was located in the cell membrane. The correlation analysis indicated that there was a negative correlation between the CmLBD2 expression and the fruit firmness during fruit ripening. CmLBD2 expression was inhibited by 1-Methylcyclopropene（1-MCP）after the fruit was processed with 1-MCP, indicating that CmLBD2 gene expression may be regulated by ethylene. In sum, LBD genes in the melon may play different roles in fruit development process, and CmLBD2 may be involved in ethylene regulation related to fruit ripening and softening after harvest.

Key words: Cucumis melo, LBD, transcriptional regulation, 1-MCP, gene expression, fruit development, bioinformatics

CHEN Qiang, ZHOU Ming-kang, SONG Jia-min, ZHANG Chong, WU Long-kun. Identification and Analysis of LBD Gene Family and Expression Analysis of Fruit Development in Cucumis melo[J]. Biotechnology Bulletin, 2023, 39(3): 176-183.

Figures/Tables 6

Fig. 1 Alignment of conserved domains of LBD family in Cucumis melo

Fig. 2 Phylogenetic tree of LBD transcription factors in C. melo and Arabidopsis

Fig. 3 Expression profile of LBD gene family in C. melo

Fig. 4 Subcellular location of CmLBD2（membrane）

Fig. 5 Correlation between the expressions of CmLBD2 and fruit firmness during postharvest storage There are 3 biological and technical replicates, the standard error is displayed on the bar graph, and lowercase letters indicate the difference among different samples. The same below

Fig. 6 Effects of 1-MCP on the expression of CmLBD2 in fruit of C. melo

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