Biotechnology Bulletin ›› 2018, Vol. 34 ›› Issue (5): 201-205.doi: 10.13560/j.cnki.biotech.bull.1985.2017-0885

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Expression of HFSC Marker in the Arbas Cashmere Goat Hair Follicle and Hair Follicle Stem Cells

NIMANTANA1, ZHANG Yan-jun1, LIU Dong-jun2, LI Jin-quan1   

  1. 1. College of Animal Science,Inner Mongolia Agricultural University,Huhehot 010018;
    2. Key Laboratory of Mammalian Reproductive Biology and Biotechnology of Ministry of Education,Inner Mongolia University,Huhehot 010070
  • Received:2017-10-22 Online:2018-05-26 Published:2018-06-07

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Abstract: This study is aimed to detect the expression of widely-used hair follicle stem cell（HFSC）markers in the hair follicle of Arbas Cashmere goat,and to provide experimental evidence for further research on histology and cytology of hair follicle in Inner Mongolia Cashmere goat. Using Arbas Cashmere goat as study object,frozen section and immunohistochemistry of Krt15,Krt19,CD34 and Sox9 were performed. Results showed that the above-mentioned four markers all expressed in the outer root sheath;Krt15 and Krt19 positively expressed in the bulge region and non-contiguously in the suprabulbar region;CD34 had a strong expression in the bulge and suprabulbar region;Sox9 expression focused in the bulge region. Conclusively,Krt15,Krt19,CD34 and Sox9 all expressed in the bulge region of the hair follicle,thus they can be used as special markers of Arbas Cashmere goat hair follicle stem cells from bulge region according to their expressed location.

Key words: Arbas Cashmere goat, frozen section, cellular immunohistochemistry, identification marker