Fermentative Production of Rhamnolipid Using Waste Oil from Range Hood as Substrate

doi:10.13560/j.cnki.biotech.bull.1985.2017-0736

Abstract

Abstract: Rhamnolipid（RL）is a kind of anionic biosurfactant with great potential and could be applied in the fields of petroleum,food,agriculture,and daily chemicals industry. This study explores the feasibility of producing rhamnolipid by fermentation with waste oil from range hood. Pseudomonas aeruginosa WB505 was used as the starting strain,and by which the yield of rhamnolipid in 7 L fermenter reached 12.3±0.52 g/L. Matrix-assisted laser desorption time-of-flight mass spectrometry was applied to analyze the composition of produced RL. Results showed that the main contents were Rha-C₁₀-C₁₀ and Rha₂-C₁₀-C₁₀,and the total abundance of mono-rhamnolipid and di-rhamnolipid were 49.7% and 50.3%,respectively. Critical micelle concentration（CMC）of the produced RL was 45.0 mg/L and it reduced the surface tension of water from 60.5±0.81 mN/m to 25.3±0.68 mN/m. E24 for six kinds of common hydrocarbons were higher than 60%,and E24 for benzene reached 80.3±0.85%. Therefore,using waste oil from range hood as substrate for RL production can reduce the capital cost of substrate,and provides a recycling treatment for waste oil from range hood.

Key words: biosurfactant, rhamnolipid, Pseudomonas aeruginosa, waste oil from range hood, critical micelle concentration

ZHANG Jing-bo, WU Jian-rong, JIANG Yun, Yang Di, ZHAN Xiao-bei. Fermentative Production of Rhamnolipid Using Waste Oil from Range Hood as Substrate[J]. Biotechnology Bulletin, 2018, 34(5): 195-200.

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