Cloning and Expression of EsABCG25 from Eutrema salsugineum

doi:10.13560/j.cnki.biotech.bull.1985.2018-0020

Abstract

Abstract: This work is aimed to reveal structure of ABCG25 and explore its response to drought,salinity and temperature stresses in Eutrema salsugineum. A full-length cDNA of member gene in ATP-binding cassette transporters（ABCG）family was cloned from E. salsugineum（Shandong ecotype）using silicon cloning and RT-PCR methods,and named as EsABCG25 with GenBank accession number of KY111263. The full length of EsABCG25 was 2154 bp and it contained a complete ORF with 1983 bp and encoded 660 amino acids. EsABCG25 was located at chromosome 5,with 4 exons and 3 introns. EsABCG25 had a putative nucleotide binding domain（NMD）and a putative transmembrane domain（TMD）. Alignment and phylogenetic analysis found that EsABCG25 was the closest to the ABCG25 from Brassica rapa. High-throughput sequencing indicated that EsABCG25 reached the highest expression in the stem of E. salsugineum and the lowest expression in rosette leaves,and alternative splicing events were detected in all tissues. Real-time PCR assay indicated that EsABCG25 was induced by many kinds of stresses,especially ABA stress. From above,EsABCG25 has typical characteristics of ABCG gene family. Its expression variedat different tissues,was induced by stress,and may be closely associated with the stress tolerance of E. salsugineum.

Key words: Eutrema salsugineum, ABCG25, bioinformatics, real-time PCR, expression analysis

Songbuerbatu CHEN Yue CHEN Ning-mei TANG Shuai XU Xiao-jing. Cloning and Expression of EsABCG25 from Eutrema salsugineum[J]. Biotechnology Bulletin, 2018, 34(7): 108-118.

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