Revealing Hybridization of Brassicaceae Based on Comparative Genomics

doi:10.13560/j.cnki.biotech.bull.1985.2018-0338

Abstract

Abstract: Hybridization is one of the sources for the evolutionary dynamics of species. It helps to rapidly accumulate genetic variation,to enhance the genetic diversity of the population,and to promote the production of new species. Therefore,it is of great significance to identify ancient and recent crosses in different species groups. Identification methods of hybridization event may employ a variety of features,including traditional genetic phenotypes,metabolites,chromosome numbers,and molecular phenotypes of gene levels provided by population genetics and phylogenetic genomics. In this study,28 Brassicaceae species and two species of other family were selected as research materials,and comparative genomics and phylogenetic genomics in the chloroplast and nuclear genes in combination with molecular phenotypic information were used to identify the Brassicaceae hybrid events. The results show that：1. Based on the results of mapping single species trees to species trees,Brassicaceae species are mainly classified into two groups：one is that a sister group is relatively concentrated and represented by Arabidopsis thaliana;another is that a sister group is relatively scattered,suggesting there are multiple sources of genes,represented by Lunaria annua. 2. Further,by phylogenetic analysis of chloroplast and nuclear genes,we believe that L. annua might be a new hybrid species,39.1% of its genes are most closely related to the parental C group genes;38.5% of the genes are the closest to the genes of non C groups. In particular,the No. 10 node from the common ancestors of the B and C binding groups（accounting for 3%）supports itself as a maternal source of L. annua. 3. By the functional enrichment analysis of the key evolutionary nodes of the parents,we find that the male parent is mainly enriched in biodegradation,secondary metabolites and RNA regulation,indicating that it may regulate biological degradation and secondary metabolic processes to adapt to the stress environment via RNA;maternal enriched in the metabolic pathways,especially carbohydrates,nucleic acid metabolism,etc. indicating that it may obtain relative competitive advantage by enhancing its own metabolism.

Key words: Brassicaceae, hybridization, comparative genomics

FAN Xiao-meng, QI Ji. Revealing Hybridization of Brassicaceae Based on Comparative Genomics[J]. Biotechnology Bulletin, 2018, 34(7): 126-137.

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