[1] 俞发荣, 李登楼. 有机磷农药对人类健康的影响及农药残留检测方法研究进展[J]. 生态科学, 2015, 34(3):197-203. [2] 虞云龙, 盛国英, 傅家谟. 一株农药降解菌的分离与鉴定[J]. 华南理工大学学报, 1996(S1):192-196. [3] 陈亚丽, 张先恩, 刘虹, 等. 甲基对硫磷降解菌Pseudomonas sp. WBC-3的筛选及其降解性能的研究[J]. 微生物学报, 2002, 42(4):490-497. [4] Dong YJ, Bartlam M, Sun L, et al.Crystal structure of methyl parathion hydrolase from Pseudomonas sp. WBC-3[J]. Journal of Molecular Biology, 2005, 353(3):655-663. [5] Carr R, Alexeeva M, Dr AE, et al.Directed evolution of an amine oxidase possessing both broad substrate specificity and high enantioselectivity[J]. Angewandte Chemie, 2003, 42(39):4807-4810. [6] Kim JH, Choi GS, Kim SB, et al.Enhanced thermostability and tolerance of high substrate concentration of an esterase by directed evolution[J]. Journal of Molecular Catalysis B Enzymatic, 2004, 27(4):169-175. [7] Böttcher D, Bornscheuer UT.Protein engineering of microbial enzymes[J]. Current Opinion in Microbiology, 2010, 13(3):274-282. [8] Dwyer MA, Looger LL, Hellinga HW.Computational design of a biologically active enzyme[J]. Science, 2004, 304(5679):1967-1971. [9] Khoury GA, Fazelinia HChin JW.Computational design of candida boidinii xylose reductase for altered cofactor specificity[J]. Protein Science, 2009, 18(10):2125-2138. [10] Chen CY, Georgiev I, Anderson AC, et al.Computational structure-based redesign of enzyme activity[J]. Proceedings of the National Academy of Sciences of the United States of America, 2009, 106(10):3764-3769. [11] Röthlisberger D, Khersonsky O, Wollacott AM, et al.Kemp elimination catalysts by computational enzyme design[J]. Nature, 2008, 453(7192):190-195. [12] Jiang L, Althoff EA, Clemente FR, et al.De novo computational design of retro-aldol enzymes[J]. Science, 2008, 319(5868):1387-1391. [13] Siegel JB, Zanghellini A, Lovick HM, et al.Computational design of an enzyme catalyst for a stereoselective bimolecular diels-alder reaction[J]. Science, 2010, 329(5989):309-313. [14] Toscano MD, Woycechowsky KJ, Hilvert D.Minimalist active-site redesign:teaching old enzymes new tricks[J]. Angewandte Chemie International Edition, 2007, 46(18):3212-3236. [15] Ponting CP.Biological function in the twilight zone of sequence conservation[J]. Bmc Biology, 2017, 15(1):1-9. [16] Glaser F, Pupko T, Paz I, et al.Consurf:identification of functional regions in proteins by surface-mapping of kphylogenetic information[J]. Bioinformatics, 2003, 19(1):163-164. [17] Wilkins A, Bachman B, Erdin S, et al.The use of evolutionary patterns in protein annotation[J]. Current Opinion in Structural Biology, 2012, 22(3):316-325. [18] Sankararaman S, Kolaczkowski B, Sjölander K.INTREPID:a web server for prediction of functionally important residues by evolutionary analysis[J]. Nucleic Acids Research, 2009, 37(Web Server issue):390-395. [19] Hopf TA, Schärfe CPI, Rodrigues JPGLM, et al.Sequence co-evolution gives 3D contacts and structures of protein complexes[J]. Elife, 2014, 3(6):380-389. [20] Engqvist MK, Mcisaac RS, Dollinger P, et al.Directed evolution of Gloeobacter violaceus rhodopsin spectral properties[J]. Journal of Molecular Biology, 2015, 427(1):205-220. [21] Wu NF, Deng MJ, Shi XY, et al.Isolation, purification and characterization of a new organphosphorus hydrolase OPHC2[J]. Chinese Science Bulletin, 2004, 49(3):268-272. [22] Xie J, Zhao Y, Zhang H, et al.Improving methyl parathion hydrolase to enhance its chlorpyrifos-hydrolysing efficiency[J]. Letters in Applied Microbiology, 2013, 58(1):53-59. [23] Chen K Q, Arnold F H.Enzyme engineering for nonaqueous solvents:random mutagenesis to enhance activity of subtilisin E in polar organic media[J]. Biotechnology, 1991, 9(11):1073-1077. [24] 张秀艳, 何国庆, 陈启和. 理性和非理性蛋白质设计策略在酶工程中应用[J]. 科技通报, 2007, 23(2):191-97. |