Embryonic Callus Induction of Sophora davidii and Their Somatic Embryogenesis and Germination

doi:10.13560/j.cnki.biotech.bull.1985.2018-0828

Abstract

Abstract: This work is to investigate the effects of different factors on embryonic callus induction,somatic embryogenesis and somatic embryo germination from the hypocotyl and cotyledon of Sophora davidii. Having B₅and MS as the basic medium,the inductions of 2,4-D,6-BA and TDZ on embryonic callus from the hypocotyl and cotyledon of S. davidii,the proliferation of the embryonic callus in the MS medium supplied with different concentration of 2,4-D,and the effects of ABA on somatic embryogenesis were studied. The results showed that the embryonic callus induction from the hypocotyl more easily occurred than from cotyledon. The optimal medium for the embryo callus induction for two explants were MS+ 2.0 mg/L 2,4-D + 0.5 mg/L TDZ + 0.5 mg/L 6-BA,the induction rate was 77.3% and 41.0% respectively. The 15 mg/L ABA + 0.2 mg/L 2,4-D + 2.0 mg/L 6-BA was conducive to the somatic embryogenesis. The somatic embryo germination rate was up to 80% in the medium of 1/3 MS+ 0.2 mg/L NAA+0.1 mg/L 6-BA+2.0 g/L activated carbon+25 g/L sucrose+7g/L agar. The survival rate of transplanted seedlings reached 90%. In sum,the types of the explants and medium may affect the induction of embryonic callus,for which hypocotyl was better than cotyledon. MS medium is more suitable for initiating cell dedifferentiation to generate callus. 2,4-D may regulate the proliferation and maintenance of embryonic callus,and ABA is favorable for somatic embryogenesis.

Key words: Sophora davidii, embryonic callus, somatic embryogenesis, somatic embryo germination

WU Li-fang, WEI Xiao-mei, LU Wei-dong. Embryonic Callus Induction of Sophora davidii and Their Somatic Embryogenesis and Germination[J]. Biotechnology Bulletin, 2019, 35(4): 13-19.

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