Creation of Chimeric Protein Vip3AaAdAa in Bacillus thuringiensis and Analysis of Its Insecticidal Activity

doi:10.13560/j.cnki.biotech.bull.1985.2018-0929

Abstract

Abstract:

Vip3A-type insecticidal proteins are a new class of insecticidal proteins. There is no homology in amino acid sequences with well-studied protein Cry,and the insecticidal sites are not competitive with insect receptor binding sites of Cry proteins. Homologous recombinant technique was used to study the insecticidal activity of Vip3Aa39 and inactive Vip3Ad against Spodoptera exigua. Three vip3AaAdAa chimeric genes were successfully created and were transformed into Escherichia coli to express chimeric protein Vip3AaAda1,Vip3AaAda2,and Vip3AaAda3. The insecticidal activities of these 3 chimeric proteins against S. exigua were determined. The results showed that the 3 chimeric proteins presented high insecticidal activities against S. exigua,the LC₅₀ of Vip3AaAda3 protein was 1.4 times higher than that of Vip3Aa39,and the LC₅₀ of Vip3AaAda1 protein was 2.7 times higher than that of Vip3Aa39. The insecticidal activities of chimeric proteins Vip3AaAda1 and Vip3AaAda3 were lower than that of Vip3Aa39,and the insecticidal activity of Vip3AaAdAa2 was not significantly different from that of Vip3Aa39.

Key words: Bacillus thuringiensis, chimeric protein, Spodoptera exigua, insecticidal activity

YU Shuang, LI Shuai, LI Hai-tao, LIU Rong-mei, GAO Ji-guo. Creation of Chimeric Protein Vip3AaAdAa in Bacillus thuringiensis and Analysis of Its Insecticidal Activity[J]. Biotechnology Bulletin, 2019, 35(4): 51-56.

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