Abstract: In order to study the distribution,drug resistance and species of multidrug-resistant bacteria（MDRB）in the bathing beach,MDRB were screened by resistance screening plates,and their sensitivities to 15 common antibiotics were determined by K-B paper method,and the species of MDRB were identified by 16s rDNA sequence homology alignment. The results showed that 60（22.1%）MDRB were screened from 271 cultivable seawater bacteria,and their resistance rates to 15 drug-sensitive papers ranged from 31.6% to 81.7%;their resistance rates to sulfonamides,aminoglycosides and fosfomycin were high,and the highest（81.7%）to streptomycin. Correlation analysis between streptomycin,tetracycline-resistant genes and drug-resistant phenotypes demonstrated that the drug resistance of MDRB was significantly correlated with the detection rate of drug-resistant genes. The 60 strains of MDRB belonged to 24 genera,of which the genusStenotrophil accounted for the largest proportion（16%）,followed by Microbacterium（13%）,Pseudomonas（8%）,Staphylococcus（8%）. The experimental results reveal that the MDRB pollution in Qingdao bathing beach is serious and rich in diversity,and the MDRB present strong resistances to common antibiotics.

Key words: bathing beach, bacteria, resistance rate, MDRO