Biotechnology Bulletin ›› 2019, Vol. 35 ›› Issue (6): 16-23.doi: 10.13560/j.cnki.biotech.bull.1985.2018-0891

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Cloning and Expression Analysis of the PpNAC72 in Peach

WU Xiao-qi, FENG Tao, LIU Yan-jun, JIAO Si-peng, SUN Pan, YANG Jing-hui   

  1. College of Horticulture,Tianjin Agricultural University,Tianjin 300384
  • Received:2018-10-17 Online:2019-06-26 Published:2019-07-08

Abstract: The aims are to clone the PpNAC72 from the peach sepecies “Jinliuzaohong”,to analyze the expression profile in different development stages,and to explore its role in the peach growth. RT-PCR was used to clone the PpNAC72,and subsequently to quantitatively analyze its expression profile and bioinformatics. The results showed that the PpNAC72(GenBank accession number:MH627940)had an open reading frame sequence of 1 050 bp,encoding 349 amino acids,an isoelectric point of 8.34,and a NAM domain with typical NAC family structure. Amino acid homology analysis demonstrated that PpNAC72 had high similarity to the reported NAC of other plants,and the NAC protein had the highest homology with sweet cherry. The results of qRT-PCR revealed that PpNAC72 was expressed in different degrees in peach organs,and the expression level was the highest in fruits. The expression level was also significantly varied in different periods,and the expression level was the highest in the middle and late growth stages of peach fruit. The PpNAC72 might be involved in the maturation of peach as a transcription factor.

Key words: peach, PpNAC72, mature, expression analysis