Biotechnology Bulletin ›› 2019, Vol. 35 ›› Issue (6): 24-31.doi: 10.13560/j.cnki.biotech.bull.1985.2018-1101

Previous Articles     Next Articles

Transcriptome Sequencing and Analysis of Xanthoceras sorbifolia Bunge Fruit

ZHAO Yang-yang, GUO Yu-xiao, ZHANG Ling-yun   

  1. Key Laboratory of Forestry Silviculture and Conservation of the Ministry of Education,Beijing Forestry University,Beijing 100083
  • Received:2018-12-29 Online:2019-06-26 Published:2019-07-08

Abstract: In order to study the expressions of functional genes in the transcriptome of Xanthoceras sorbifolia Bunge fruit,the RNA-Seq technology was used to sequence Xanthoceras sorbifolia Bunge fruit in different stag. The sequencing results showed that a total of 68 298 Unigene sequences after sequencing and assembly were obtained from the samples in 2 stages,and 24 691 Unigene sequences were annotated,accounting for 36.15%. Based on GO database,the 14 766 Unigene sequences were divided into 3 categories of cellular component,molecular function and biological process and 54 function groups. The 13 994 Unigene sequences were grouped into 25 functional categories based on KOG database. Based on KEGG metabolic pathway database,8 284 Unigene sequences of X. sorbifolia Bunge fruit may be classified into 127 metabolic pathways. And 11 732 SSR(Simple Sequence Repeats)loci were discovered in X. sorbifolia Bunge fruit transcriptome. Most of them were single nucleotide SSR,accounting for 60.85%. This study provides the foundation and reference for molecular biology study in X.sorbifolia Bunge fruit.

Key words: Xanthoceras sorbifolia Bunge, fruit, transcriptome sequencing, SSR marker