Differentially Physiological and Biochemical Responses of Grape Cells to Contacted Co-culture Endophytic Fungal Strains

doi:10.13560/j.cnki.biotech.bull.1985.2019-0211

Abstract

Abstract:

Endophytic fungal strains were classified according to the differentially physiological and biochemical responses of grape cells during their co-culturing with endophytic fungi,which may be conducive to screening endophytic fungal resources with varied application values. Grape callus（Vitis vinifera L. cultivar：Gamay）and 47 endophytic fungal strains from 18 genera isolated from grape varieties Rose honey,Cabernet Sauvignon and Xiahei were chosen as experimental materials to construct endophytic fungi-grape cell contacted co-culture system. Further the physiological and biochemical effects of these endophytic fungal strains on grape cellular,growth,anthocyanin contents,phenylalanine ammonia-lyase（PAL）activities and others were analyzed. Ours results indicated that different endophytic fungal strains initiated different degrees of impacts on the co-cultured grape cells in growth,anthocyanin contents and PAL activity. Among all those used endophytic fungal strains,20 conferred less damages to grape cells,6 significantly promoted the co-cultured grape cells in growth,11 significantly promoted the co-cultured grape cellular PAL activity,and 5 promoted the co-cultured grape cells’ anthocyanin contents. Beside the development of candidate endophytic fungal strains,this research provided a methodology for purposely screening candidate endophytic fungal resources.

Key words: gape cells, endophytic fungal resource, physiological and biochemical response, contacted co-culture system, quality regulation

YU Man, CHEN Jing-chao, QU Jin-zhuo, LIU Fang, PAN Xiao-xia, YANG Ming-zhi. Differentially Physiological and Biochemical Responses of Grape Cells to Contacted Co-culture Endophytic Fungal Strains[J]. Biotechnology Bulletin, 2019, 35(8): 34-41.

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