Screening,Identification and Antifungal Properties of a Bacterium with Antagonistic Activities Against Mycotoxin-producing Aspergillus spp.

doi:10.13560/j.cnki.biotech.bull.1985.2019-0074

Abstract

Abstract:

To obtain the antagonistic strains against Aspergillus ochraceus and Aspergillus flavus and to fully exploit the beneficial microbial resources,efficient antagonistic strains against Aspergillus ochraceus and Aspergillus flavus were isolated and purified from plantation soil and Vigna angularis. Then,the morphology,physiological and biochemical reactions,16S rDNA phylogenetic analysis and specific PCR analysis were applied for the identification of the antagonistic bacteria. Furthermore,the bacteriostatic stability of the fermentation supernatant,etc. were studied. Strain SC-B15,isolated from the plantation soil,had strong antagonistic effects on A. ochraceus and A. flavus growth,and the bacteriostatic rates against A. ochraceus and A. flavus reached 47.30±13.17% and 52.44±2.78%,respectively. SC-B15 was identified as Bacillus amyloliquefaciens. The inhibition zone diameters of the supernatant against A. ochraceus and A. flavus were 15.03±2.66 mm and 13.95±2.62 mm,respectively. Meanwhile,the bacteriostatic substances remained stable at 20-40℃ and pH=6-10,and were insensitive to trypsin and papain. The indicated strains appeared abnormal changes of cavitation,swelling and deformation with microscope. In sum,B. amyloliquefaciens SC-B15,isolated from plantation soil,and its supernatant,efficiently inhibit the A. ochraceus and A. flavus growth and demonstrate certain development application value as antifungal agent in the foodstuff and feedstuff.

Key words: Bacillus amyloliquefaciens, antifungal activity, Aspergillus flavus, Aspergillus ochraceus

LIU Duan-mu, WU Yi, LIU Yun, LIANG Zhi-hong. Screening,Identification and Antifungal Properties of a Bacterium with Antagonistic Activities Against Mycotoxin-producing Aspergillus spp.[J]. Biotechnology Bulletin, 2019, 35(8): 42-50.

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