Preparation of Anti-lung Cancer Peptides by Enzymatic Hydrolysis of Protein from Enteromorpha clathrata

doi:10.13560/j.cnki.biotech.bull.1985.2019-0338

Abstract

Abstract: In order to exploit abundant proteins in the green algae Enteromorpha clathrata,protein from E. clathrata was hydrolyzed by the papain enzyme,the optimal hydrolysis process conditions were solid/liquid ratio of 1∶25,enzyme dose of 1 250 U/g protein,hydrolysis temperature of 45.7℃,hydrolysis pH of 7.25 and hydrolysis duration of 120 min. Under these conditions,polypeptides of different molecular weight range were obtained by ultrafiltration,and their anti-lung cancer effects were evaluated preliminarily at the cellular level of HUVEC（human umbilical vein endothelial cell）,A549,H446 and H460. According to the results,the oxidation resistance,proliferation and tubule formation of HUVEC treated with 2-6 kD polypeptide from E. clathrata were inhibited,and the proliferation,mobility,cell cycle of all 3 tested lung cancer cells H446,H460 and A549 were inhibited at varied level,and the cells apoptosis were induced. The 2-6 kD proteins demonstrated significantly better inhibitory effect on H446 and H460 than that on A549. In conclusion,this study proves the feasibility of gaining natural anti-tumor small-molecule peptides by enzymolysis protein from E. clathrata,and provides a novel way to explore the high value utilization of protein resources from E. clathrata.

Key words: Enteromorpha clathrata, enzymatic hydrolysis, peptide, lung cancer

DUAN Xun-wei, XIAO Gui-qing, WANG Li-xing, WU Wen-lin, DAI Cong-jie, DONG Le. Preparation of Anti-lung Cancer Peptides by Enzymatic Hydrolysis of Protein from Enteromorpha clathrata[J]. Biotechnology Bulletin, 2019, 35(8): 246-252.

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