Abstract: By checking regulatory elements and marker genes in common genetic transformation vectors of GM maize lines in relevant databases and combining it with the current domestic and foreign standard methods for the detection of GM,the strategy of screening transgenic maize by real-time fluorescent PCR method was established based on the P-CaMV35S,T-NOS,Bar gene,Pat gene,Cp4-epsps gene,NPT II gene,cry1A gene,P-Rice actin,and phy structure specific fragments,and 32 varieties of transgenic maize were tested in the screening. The results showed that the screening strategy covered 30 independent trait GMO events. Meanwhile,a plasmid molecule containing maize internal standard gene and 9 target elements/gene sequences was constructed. It was proved that the plasmid molecule had favorable substitution and could be used as a positive control in conjunction with this detection method.

Key words: genetically modified maize, screening, GMO event, detection