The Study on the Adsorption Properties of Six Proteins on Biological Magnetic Nanobeads

doi:10.13560/j.cnki.biotech.bull.1985.2020-0032

Abstract

Abstract: In recent years,magnetic nanobeads have received extensive attentions in the fields of biotechnology and biomedicine;however,there is often non-specific adsorption between proteins and biological magnetic nanobeads. Therefore,the relationship between the structure and physicochemical properties of proteins and magnetic bead adsorption was studied from the perspective of proteins. Using two types of biological magnetic nanobeads,the adsorption specificities of 6 different proteins on the surfaces of magnetic beads were studied. Each protein’s structure was analyzed by protein analysis software,and the structure and physicochemical property of different proteins were obtained. The relationship between protein properties and the adsorption specificity of the magnetic bead was analyzed. There was non-specific adsorption for different proteins on the surfaces of magnetic nanobeads,and the size of the protein was not significantly related to the ability of magnetic beads to adsorb proteins. As the β-sheet ratio of protein decreased,the non-specific adsorption of protein on the surfaces of magnetic beads also decreased. Human Tumor Necrosis Factor alpha（TNFα）with the largest β-sheet ratio had the most non-specific adsorption on the magnetic beads,and a single protein band was obtained by the adsorption. Human Annexin A5 with the smallest β-sheet ratio had the least non-specific adsorption on the magnetic beads. After Annexin A5 was fused with Enhanced Green Fluorescent Protein（EGFP）,the β-sheet ratio was increased,and the non-specific adsorption by magnetic beads also increased. In conclusion,there is no obvious correlation between the protein size and the non-specific adsorption on the surfaces of magnetic beads. The non-specific adsorption capacity of magnetic beads to proteins is positively related to the β-sheet ratio of the protein structure. Protein secondary structure is an important factor for the non-specific adsorption of proteins to magnetic beads.

Key words: magnetic nanobeads, different proteins, non-specific adsorption, β-sheet ratio

WANG Meng-hui, GAO Ying, LI Jia-huang, HUA Zi-chun. The Study on the Adsorption Properties of Six Proteins on Biological Magnetic Nanobeads[J]. Biotechnology Bulletin, 2020, 36(6): 208-214.

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