Secondary Metabolic Pathway Mining and Pan-genome Analysis of Bacillus coagulans

doi:10.13560/j.cnki.biotech.bull.1985.2020-0097

Abstract

Abstract:

This study was to further understand the genome of Bacillus coagulans and to explore its secondary metabolic pathways. The genomes of 33 B. coagulans were downloaded from the NCBI database and analyzed by pan-genomic analysis software and secondary metabolite mining software. The genome size of 33 strains of B. coagulans was between 2.059 47-3.694 84 Mbp and the GC content ranged from 46.2% to 47.5%. Pan-genome analysis of 11 strains of B. coagulans with complete genome level revealed that the pan-genome contained 5 899 cluster genes,the core genome consisted of 2 152 cluster genes,and 2 255 strain-specific genes were identified. Based on the analysis of secondary metabolite synthesis gene clusters,79 secondary metabolite gene clusters of 8 groups were found in the 33 B. coagulans genomes. The major synthetic gene clusters were bacteriocins,lactones and sugars. In brief,the pan-genome and core genome sizes of B. coagulans and their secondary metabolites through can be predicted in this study,which is conducive to understanding B. coagulans and providing relevant information further better utilization of this strain.

Key words: Bacillus coagulans, pan-genome, secondary metabolic gene cluster

QIAN Wen-jiang, WANG Bu-qing, LI Wei-xi, YANG Xue-miao, LIU Hong-wei, ZHANG Li-ping. Secondary Metabolic Pathway Mining and Pan-genome Analysis of Bacillus coagulans[J]. Biotechnology Bulletin, 2020, 36(10): 88-98.

Figures/Tables 9

References 29

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物种	菌株	组装水平	基因组大小/Mb	GC/%	基因	蛋白	描述	登录号
Bacillus coagulans	S-lac	CompleteGenome	3.69484	46.2	3 559	3247	信息缺失	CP011939.1
	IDCC1201	CompleteGenome	3.66421	46.2	3 537	3 237	从韩国华城麦芽中分离	CP035305.1
	LA204	CompleteGenome	3.64257	46.2	3 618	3 327	从武汉玉米田土壤中分离	CP025437.1
	DSM2314	CompleteGenome	3.62865	46.2	3 515	3 231	从根际分离	CP033687.1
	HM-08	CompleteGenome	3.62464	46.3	3 439	3 192	从鸡肠道中分离	CP010525.1
	R11	CompleteGenome	3.60275	46.3	3 568	3 228	从中国云南泥土中分离	CP026649.1
	BC-HY1	CompleteGenome	3.59908	46.2	3 427	3 181	从猪消化道中分离	CP017888.1
	36D1	CompleteGenome	3.55223	46.5	3 414	3 140	信息缺失	CP003056.1
	2-6	CompleteGenome	3.07308	47.3	3 062	2 778	信息缺失	CP002472.1
	LBSC	Chromosome	3.6359	46.3	3 627	3 332	从印度纳西克泥土中分离	CP022701.1
	DSM 1	Contig	3.01804	47.2	3 039	2 779	信息缺失	ALAS00000000.1
	DSM 1	scaffold	2.95252	47.3	3 124	2 788	信息缺失	FQUB00000000.1
	ATCC7050	Contig	2.94782	47.3	3 028	2 754	信息缺失	ATUM00000000.1
	ZB29	scaffold	3.64647	46.2	3 660	3 213	从印度孟买牛奶中分离	QPFA00000000.1
	XZL9	Contig	3.42411	46.5	3 275	3 084	从中国济南泥土中分离	ANAP00000000.1
	NL01	Contig	3.50142	46.2	3 426	3 176	从中国南京泥土中分离	LBMQ00000000.1
	AF24-19	scaffold	2.9104	47.4	2 958	2 703	从人体粪便中分离	QRUW00000000.1
	MGYG-HG UT-00191	scaffold	2.9104	47.4	2 956	2 701	从人体肠道中分离	CABJCT000000000.1
	P38	Contig	3.37432	46.4	3 303	3 098	从中国泥土中分离	JSVI00000000.1
	AF24-21	scaffold	2.89159	47.5	2 890	2 699	从人体粪便分离	QRUS00000000.1
	Unique IS-2	Contig	3.44669	46.4	3 322	3 128	从被人体粪便污染的土壤中分离	JZDH00000000.1
	CSIL1	scaffold	3.46623	46.3	3 358	3 121	信息缺失	AXVW00000000.1
	HS243	Contig	3.4563	46.4	3 429	2 732	从印度浦那马哈拉施特拉邦商业食品中分离获取	NFZN00000000.1
	GBI-30,6086	Contig	3.45862	46.4	3 384	3 133	从冻干粉中分离获得	JPSK00000000.1
	B4100	Scaffold	3.42452	46.5	3 350	3 108	从意大利乳清干酪中分离	LQYH00000000.1
	GED7749B	Scaffold	3.31243	46.5	3 150	3 017	从阴道中分离获取	LRPN00000000.1
	B4098	Scaffold	2.9287	47.4	2 952	2 705	从中国番茄罐头中分离	LQYG00000000.1
	B4096	scaffold	3.3136	46.5	3 184	2 994	从中国番茄罐头中分离	LQYJ00000000.1
	MA-13	Contig	2.05947	46.9	2 064	1 804	从农业废弃物中分离	SMSP00000000.1
	XZL4	scaffold	2.86628	47.4	2 818	2 558	信息缺失	AFWM00000000.1
	B4099	scaffold	3.27533	47.1	3 287	2 999	从印度咖喱中分离	LQYI00000000.1
	H-1	Contig	2.86288	47.3	2 788	2 513	从上海泥土中分离	ANAQ00000000.1
	ATCC 7050	CompleteGenome	3.367	46.9	3 405	3 253	从爱荷华州乳制品中分离	CP009709.1

物种	菌株	组装水平	基因组大小/Mb	GC/%	基因	蛋白	描述	登录号
Bacillus coagulans	S-lac	CompleteGenome	3.69484	46.2	3 559	3247	信息缺失	CP011939.1
	IDCC1201	CompleteGenome	3.66421	46.2	3 537	3 237	从韩国华城麦芽中分离	CP035305.1
	LA204	CompleteGenome	3.64257	46.2	3 618	3 327	从武汉玉米田土壤中分离	CP025437.1
	DSM2314	CompleteGenome	3.62865	46.2	3 515	3 231	从根际分离	CP033687.1
	HM-08	CompleteGenome	3.62464	46.3	3 439	3 192	从鸡肠道中分离	CP010525.1
	R11	CompleteGenome	3.60275	46.3	3 568	3 228	从中国云南泥土中分离	CP026649.1
	BC-HY1	CompleteGenome	3.59908	46.2	3 427	3 181	从猪消化道中分离	CP017888.1
	36D1	CompleteGenome	3.55223	46.5	3 414	3 140	信息缺失	CP003056.1
	2-6	CompleteGenome	3.07308	47.3	3 062	2 778	信息缺失	CP002472.1
	LBSC	Chromosome	3.6359	46.3	3 627	3 332	从印度纳西克泥土中分离	CP022701.1
	DSM 1	Contig	3.01804	47.2	3 039	2 779	信息缺失	ALAS00000000.1
	DSM 1	scaffold	2.95252	47.3	3 124	2 788	信息缺失	FQUB00000000.1
	ATCC7050	Contig	2.94782	47.3	3 028	2 754	信息缺失	ATUM00000000.1
	ZB29	scaffold	3.64647	46.2	3 660	3 213	从印度孟买牛奶中分离	QPFA00000000.1
	XZL9	Contig	3.42411	46.5	3 275	3 084	从中国济南泥土中分离	ANAP00000000.1
	NL01	Contig	3.50142	46.2	3 426	3 176	从中国南京泥土中分离	LBMQ00000000.1
	AF24-19	scaffold	2.9104	47.4	2 958	2 703	从人体粪便中分离	QRUW00000000.1
	MGYG-HG UT-00191	scaffold	2.9104	47.4	2 956	2 701	从人体肠道中分离	CABJCT000000000.1
	P38	Contig	3.37432	46.4	3 303	3 098	从中国泥土中分离	JSVI00000000.1
	AF24-21	scaffold	2.89159	47.5	2 890	2 699	从人体粪便分离	QRUS00000000.1
	Unique IS-2	Contig	3.44669	46.4	3 322	3 128	从被人体粪便污染的土壤中分离	JZDH00000000.1
	CSIL1	scaffold	3.46623	46.3	3 358	3 121	信息缺失	AXVW00000000.1
	HS243	Contig	3.4563	46.4	3 429	2 732	从印度浦那马哈拉施特拉邦商业食品中分离获取	NFZN00000000.1
	GBI-30,6086	Contig	3.45862	46.4	3 384	3 133	从冻干粉中分离获得	JPSK00000000.1
	B4100	Scaffold	3.42452	46.5	3 350	3 108	从意大利乳清干酪中分离	LQYH00000000.1
	GED7749B	Scaffold	3.31243	46.5	3 150	3 017	从阴道中分离获取	LRPN00000000.1
	B4098	Scaffold	2.9287	47.4	2 952	2 705	从中国番茄罐头中分离	LQYG00000000.1
	B4096	scaffold	3.3136	46.5	3 184	2 994	从中国番茄罐头中分离	LQYJ00000000.1
	MA-13	Contig	2.05947	46.9	2 064	1 804	从农业废弃物中分离	SMSP00000000.1
	XZL4	scaffold	2.86628	47.4	2 818	2 558	信息缺失	AFWM00000000.1
	B4099	scaffold	3.27533	47.1	3 287	2 999	从印度咖喱中分离	LQYI00000000.1
	H-1	Contig	2.86288	47.3	2 788	2 513	从上海泥土中分离	ANAQ00000000.1
	ATCC 7050	CompleteGenome	3.367	46.9	3 405	3 253	从爱荷华州乳制品中分离	CP009709.1

菌株	登录号	基因簇	类型	长度	起始点	终止点
S-lac	CP011939.1	Cluster1	bacteriocin	10 212	244 719	254 931
	CP011939.1	Cluster2	betalactone	28 500	421 549	450 049
	CP011939.1	Cluste3	T3PKS	41 184	1 368 873	1 410 057
IDCC1201	CP035305.1	Cluster4	bacteriocin	9 164	1 948 175	1 957 339
	CP035305.1	Cluster5	betalactone	28 500	2 124 507	2 153 007
	CP035305.1	Cluster6	bacteriocin	10 227	2 645 063	2 655 290
	CP035305.1	Cluster7	T3PKS	41 184	3 073 385	3 114 569
LA204	CP025437.1	Cluster8	LAP	23 104	89 293	112 397
	CP025437.1	Cluster9	bacteriocin	9 627	2 744 552	2 754 179
	CP025437.1	Cluster10	bacteriocin	9 476	2 817 877	2 827 353
	CP025437.1	Cluster11	betalactone	27 796	3 021 109	3 048 905
	CP025437.1	Cluster12	bacteriocin	10 227	3 511 998	3 522 225
DSM 2314	CP033687.1	Cluster13	bacteriocin	9 627	250 405	260 032
	CP033687.1	Cluster14	bacteriocin	9 476	323 727	333 203
	CP033687.1	Cluster15	betalactone	27 796	529 699	557 495
	CP033687.1	Cluster16	bacteriocin	10 227	1 018 620	1 028 847
	CP033687.1	Cluster17	LAP	23 104	1 238 486	1 261 590
HM-08	CP010525.1	Cluster18	betalactone	28 500	639 210	667 710
	CP010525.1	Cluster19	bacteriocin	10 212	834 035	844 247
R11	CP026649.1	Cluster20	bacteriocin	9 667	2 771 450	2 781 117
	CP026649.1	Cluster21	betalactone	28 570	2 954 738	2 983 308
	CP026649.1	Cluster22	bacteriocin	10 227	3 478 574	3 488 801
BC-HY1	CP017888.1	Cluster23	thiopeptide	25 017	362 914	387 931
	CP017888.1	Cluster24	bacteriocin	9 471	1 965 274	1 974 745
	CP017888.1	Cluster25	betalactone	28 381	2 194 844	2 223 225
	CP017888.1	Cluster26	terpene	21 865	2 236 543	2 258 408
	CP017888.1	Cluster27	bacteriocin	10 227	2 706 642	2 716 869
36D1	CP003056.1	Cluster28	betalactone	28 255	2 024 346	2 052 601
	CP003056.1	Cluster29	bacteriocin	10 338	2 271 308	2 281 646
2-6	CP002472.1	Cluster30	T3PKS	41 172	187 665	228 837
LBSC	CP022701.1	Cluster31	bacteriocin	9 775	138 515	148 290
	CP022701.1	Cluster32	betalactone	28 500	642 143	670 643
	CP022701.1	Cluster33	bacteriocin	10 338	836 553	846 891
DSM 1_1	FQUB01000013.1	Cluster34	T3PKS	26 788	29 638	56 426
ZB29	QPFA01000001.1	Cluster35	bacteriocin	9 352	1 563 368	1 572 720
	QPFA01000001.1	Cluster36	betalactone	28 500	1 742 415	1 770 915
	QPFA01000001.1	Cluster37	bacteriocin	10 227	2 227 614	2 237 841
AF24-19	QRUW01000037.1	Cluster38	T3PKS	25 287	1	25 288
P38	JSVI01000006.1	Cluster39	betalactone	28 246	27 219	55 465
	JSVI01000047.1	Cluster40	bacteriocin	10 059	25 667	35 726
	JSVI01000063.1	Cluster41	bacteriocin	8 694	12 239	20 933
AF24-21	QRUS01000001.1	Cluster42	saccharide	24 880	3 728	28 608
	QRUS01000001.1	Cluster43	saccharide	31 154	94 433	125 587
	QRUS01000004.1	Cluster44	saccharide	24 939	29 550	54 489
	QRUS01000004.1	Cluster45	saccharide	36 214	57 428	93 642
	QRUS01000006.1	Cluster46	saccharide	54 277	3 383	57 660
	QRUS01000007.1	Cluster47	fatty_acid	22 247	56 035	78 282
	QRUS01000008.1	Cluster48	saccharide	35 116	60 649	95 765
	QRUS01000017.1	Cluster49	saccharide	41 249	5 918	47 167
	QRUS01000018.1	Cluster50	saccharide	21 642	24 679	46 321
	QRUS01000023.1	Cluster51	saccharide	30 277	8 181	38 458
	QRUS01000024.1	Cluster52	saccharide	35 431	4 125	39 556
	QRUS01000030.1	Cluster53	saccharide	30 072	2 908	32 980
	QRUS01000039.1	Cluster54	T3PKS	25 044	1	25 045
	QRUS01000042.1	Cluster55	fatty_acid	16 223	2 469	18 692
Unique IS-2	JZDH01000003.1	Cluster56	bacteriocin	10 227	3 494	13 721
	JZDH01000012.1	Cluster57	betalactone	27 244	1	27 245
	JZDH01000012.1	Cluster58	bacteriocin	5 397	193 633	199 030
HS243	NFZN01000010.1	Cluster59	betalactone	27 523	41 565	69 088
	NFZN01000014.1	Cluster60	bacteriocin	10 227	18 875	29 102
	NFZN01000026.1	Cluster61	bacteriocin	5 525	42 873	48 398
GBI-30,6086	JPSK01000068.1	Cluster62	bacteriocin	10 227	18 640	28 867
	JPSK01000095.1	Cluster63	betalactone	16 648	1	16 649
	JPSK01000097.1	Cluster64	bacteriocin	5 435	42 803	48 238
B4100	LQYH01000015.1	Cluster65	betalactone	27 796	4 691	32 487
	LQYH01000022.1	Cluster66	bacteriocin	10 338	9 516	19 854
	LQYH01000044.1	Cluster67	thiopeptide	25 017	54 079	79 096
GED7749B	KQ955874.1	Cluster68	betalactone	15 851	1	15 852
	KQ955875.1	Cluster69	bacteriocin	10 338	49 800	60 138
	KQ955921.1	Cluster70	bacteriocin	5 337	1	5 338
	KQ955931.1	Cluster71	thiopeptide	7 473	1	7 474
B4098	LQYG01000055.1	Cluster72	T3PKS	23 277	21 835	45 112
B4096	LQYJ01000010.1	Cluster73	betalactone	23 669	17 246	40 915
	LQYJ01000039.1	Cluster74	LAP	15 866	67 480	83 346
	LQYJ01000041.1	Cluster75	T3PKS	64 83	1	64 84
B4099	LQYI01000035.1	Cluster76	terpene	12 783	8 502	21 285
	LQYI01000062.1	Cluster77	T3PKS	7 228	1	7 229
	LQYI01000073.1	Cluster78	betalactone	20 280	1	20 281
ATCC 7050	CP009709.1	Cluster79	T3PKS	41 172	2 513 409	2 554 581

菌株	登录号	基因簇	类型	长度	起始点	终止点
S-lac	CP011939.1	Cluster1	bacteriocin	10 212	244 719	254 931
	CP011939.1	Cluster2	betalactone	28 500	421 549	450 049
	CP011939.1	Cluste3	T3PKS	41 184	1 368 873	1 410 057
IDCC1201	CP035305.1	Cluster4	bacteriocin	9 164	1 948 175	1 957 339
	CP035305.1	Cluster5	betalactone	28 500	2 124 507	2 153 007
	CP035305.1	Cluster6	bacteriocin	10 227	2 645 063	2 655 290
	CP035305.1	Cluster7	T3PKS	41 184	3 073 385	3 114 569
LA204	CP025437.1	Cluster8	LAP	23 104	89 293	112 397
	CP025437.1	Cluster9	bacteriocin	9 627	2 744 552	2 754 179
	CP025437.1	Cluster10	bacteriocin	9 476	2 817 877	2 827 353
	CP025437.1	Cluster11	betalactone	27 796	3 021 109	3 048 905
	CP025437.1	Cluster12	bacteriocin	10 227	3 511 998	3 522 225
DSM 2314	CP033687.1	Cluster13	bacteriocin	9 627	250 405	260 032
	CP033687.1	Cluster14	bacteriocin	9 476	323 727	333 203
	CP033687.1	Cluster15	betalactone	27 796	529 699	557 495
	CP033687.1	Cluster16	bacteriocin	10 227	1 018 620	1 028 847
	CP033687.1	Cluster17	LAP	23 104	1 238 486	1 261 590
HM-08	CP010525.1	Cluster18	betalactone	28 500	639 210	667 710
	CP010525.1	Cluster19	bacteriocin	10 212	834 035	844 247
R11	CP026649.1	Cluster20	bacteriocin	9 667	2 771 450	2 781 117
	CP026649.1	Cluster21	betalactone	28 570	2 954 738	2 983 308
	CP026649.1	Cluster22	bacteriocin	10 227	3 478 574	3 488 801
BC-HY1	CP017888.1	Cluster23	thiopeptide	25 017	362 914	387 931
	CP017888.1	Cluster24	bacteriocin	9 471	1 965 274	1 974 745
	CP017888.1	Cluster25	betalactone	28 381	2 194 844	2 223 225
	CP017888.1	Cluster26	terpene	21 865	2 236 543	2 258 408
	CP017888.1	Cluster27	bacteriocin	10 227	2 706 642	2 716 869
36D1	CP003056.1	Cluster28	betalactone	28 255	2 024 346	2 052 601
	CP003056.1	Cluster29	bacteriocin	10 338	2 271 308	2 281 646
2-6	CP002472.1	Cluster30	T3PKS	41 172	187 665	228 837
LBSC	CP022701.1	Cluster31	bacteriocin	9 775	138 515	148 290
	CP022701.1	Cluster32	betalactone	28 500	642 143	670 643
	CP022701.1	Cluster33	bacteriocin	10 338	836 553	846 891
DSM 1_1	FQUB01000013.1	Cluster34	T3PKS	26 788	29 638	56 426
ZB29	QPFA01000001.1	Cluster35	bacteriocin	9 352	1 563 368	1 572 720
	QPFA01000001.1	Cluster36	betalactone	28 500	1 742 415	1 770 915
	QPFA01000001.1	Cluster37	bacteriocin	10 227	2 227 614	2 237 841
AF24-19	QRUW01000037.1	Cluster38	T3PKS	25 287	1	25 288
P38	JSVI01000006.1	Cluster39	betalactone	28 246	27 219	55 465
	JSVI01000047.1	Cluster40	bacteriocin	10 059	25 667	35 726
	JSVI01000063.1	Cluster41	bacteriocin	8 694	12 239	20 933
AF24-21	QRUS01000001.1	Cluster42	saccharide	24 880	3 728	28 608
	QRUS01000001.1	Cluster43	saccharide	31 154	94 433	125 587
	QRUS01000004.1	Cluster44	saccharide	24 939	29 550	54 489
	QRUS01000004.1	Cluster45	saccharide	36 214	57 428	93 642
	QRUS01000006.1	Cluster46	saccharide	54 277	3 383	57 660
	QRUS01000007.1	Cluster47	fatty_acid	22 247	56 035	78 282
	QRUS01000008.1	Cluster48	saccharide	35 116	60 649	95 765
	QRUS01000017.1	Cluster49	saccharide	41 249	5 918	47 167
	QRUS01000018.1	Cluster50	saccharide	21 642	24 679	46 321
	QRUS01000023.1	Cluster51	saccharide	30 277	8 181	38 458
	QRUS01000024.1	Cluster52	saccharide	35 431	4 125	39 556
	QRUS01000030.1	Cluster53	saccharide	30 072	2 908	32 980
	QRUS01000039.1	Cluster54	T3PKS	25 044	1	25 045
	QRUS01000042.1	Cluster55	fatty_acid	16 223	2 469	18 692
Unique IS-2	JZDH01000003.1	Cluster56	bacteriocin	10 227	3 494	13 721
	JZDH01000012.1	Cluster57	betalactone	27 244	1	27 245
	JZDH01000012.1	Cluster58	bacteriocin	5 397	193 633	199 030
HS243	NFZN01000010.1	Cluster59	betalactone	27 523	41 565	69 088
	NFZN01000014.1	Cluster60	bacteriocin	10 227	18 875	29 102
	NFZN01000026.1	Cluster61	bacteriocin	5 525	42 873	48 398
GBI-30,6086	JPSK01000068.1	Cluster62	bacteriocin	10 227	18 640	28 867
	JPSK01000095.1	Cluster63	betalactone	16 648	1	16 649
	JPSK01000097.1	Cluster64	bacteriocin	5 435	42 803	48 238
B4100	LQYH01000015.1	Cluster65	betalactone	27 796	4 691	32 487
	LQYH01000022.1	Cluster66	bacteriocin	10 338	9 516	19 854
	LQYH01000044.1	Cluster67	thiopeptide	25 017	54 079	79 096
GED7749B	KQ955874.1	Cluster68	betalactone	15 851	1	15 852
	KQ955875.1	Cluster69	bacteriocin	10 338	49 800	60 138
	KQ955921.1	Cluster70	bacteriocin	5 337	1	5 338
	KQ955931.1	Cluster71	thiopeptide	7 473	1	7 474
B4098	LQYG01000055.1	Cluster72	T3PKS	23 277	21 835	45 112
B4096	LQYJ01000010.1	Cluster73	betalactone	23 669	17 246	40 915
	LQYJ01000039.1	Cluster74	LAP	15 866	67 480	83 346
	LQYJ01000041.1	Cluster75	T3PKS	64 83	1	64 84
B4099	LQYI01000035.1	Cluster76	terpene	12 783	8 502	21 285
	LQYI01000062.1	Cluster77	T3PKS	7 228	1	7 229
	LQYI01000073.1	Cluster78	betalactone	20 280	1	20 281
ATCC 7050	CP009709.1	Cluster79	T3PKS	41 172	2 513 409	2 554 581

基因簇	最相似已知基因簇	相似度	MIBiG 数据库登录号	基因簇	最相似已知基因簇	相似度	MIBiG 数据库登录号
Cluster2	Fengycin	40%	CP011939.1	Cluster38	rhizocticin A	6%	QRUW01000037.1
Cluster4	Amylocyclicin	66%	CP035305.1	Cluster39	Fengycin	40%	JSVI01000006.1
Cluster5	Fengycin	40%	CP035305.1	Cluster41	Amylocyclicin	66%	JSVI01000063.1
Cluster8	Listeriolysin S	37%	CP025437.1	Cluster42	tauramamide	13%	QRUS01000001.1
Cluster10	Amylocyclicin	66%	CP025437.1	Cluster43	dipeptide aldehydes	11%	QRUS01000001.1
Cluster11	Fengycin	40%	CP025437.1	Cluster46	molybdenum cofactor	17%	QRUS01000006.1
Cluster14	Amylocyclicin	66%	CP033687.1	Cluster47	chejuenolide A/	7%	QRUS01000007.1
Cluster15	Fengycin	40%	CP033687.1		chejuenolide B
Cluster17	Listeriolysin S	37%	CP033687.1	Cluster51	exopolysaccharide	10%	QRUS01000023.1
Cluster18	Fengycin	40%	CP010525.1	Cluster54	rhizocticin A	6%	QRUS01000039.1
Cluster19	Amylocyclicin	66%	CP010525.1	Cluster57	Fengycin	40%	JZDH01000012.1
Cluster20	Amylocyclicin	66%	CP026649.1	Cluster59	Fengycin	40%	NFZN01000010.1
Cluster21	Fengycin	40%	CP026649.1	Cluster63	Fengycin	40%	JPSK01000095.1
Cluster24	Amylocyclicin	66%	CP017888.1	Cluster65	Fengycin	40%	LQYH01000015.1
Cluster25	Fengycin	40%	CP017888.1	Cluster66	Amylocyclicin	50%	LQYH01000022.1
Cluster26	Kanamycin	1%	CP017888.1	Cluster68	Fengycin	40%	KQ955874.1
Cluster28	Fengycin	40%	CP003056.1	Cluster69	Amylocyclicin	66%	KQ955875.1
Cluster29	Amylocyclicin	66%	CP003056.1	Cluster73	Fengycin	40%	LQYJ01000010.1
Cluster32	Fengycin	40%	CP022701.1	Cluster74	Listeriolysin S	37%	LQYJ01000039.1
Cluster33	Amylocyclicin	66%	CP022701.1	Cluster76	Kanamycin	1%	LQYI01000035.1
Cluster35	Amylocyclicin	66%	QPFA01000001.1	Cluster78	Fengycin	40%	LQYI01000073.1
Cluster36	Fengycin	40%	QPFA01000001.1	Cluster79	rhizocticin A	6%	CP009709.1