Comparative Study of Pig Duodenum Between Tissue Perfusion Culture and Static Culture

doi:10.13560/j.cnki.biotech.bull.1985.2020-0713

Abstract

Abstract:

Tissue perfusion culture and static culture are the two most common methods of intestinal tissue culture in vitro,however,it is unclear which method is more suitable for the study of gut hormone secretion. This research used pig duodenum as the research object and chose L-arginine as stimulus. Firstly,we compared the effects of the 2 in vitro methods on cholecystokinin and lactate dehydrogenase secretion stimulated with L-arginine;furthermore,we made a comparison of the CCK levels in the duodenum treated with L-arginine in vivo,aiming to determine which method was more suitable for the study of gut hormone secretion in vitro. Results showed that CCK concentration fluctuated steadily and LDH concentration decreased gradually with the increase of time in the tissue perfusion culture;while in static culture,CCK and LDH concentration increased over time. In addition,the secretion of CCK in the tissue perfusion culture was very similar to that in blood. Above results demonstrate that the tissue perfusion culture is more suitable for the study of gut hormone secretion in vitro.

Key words: tissue perfusion culture, tissue static culture, L-arginine, CCK, LDH

WANG Lü-yang, KANG Cui-cui, FENG Jiang-yin, DING Li-ren, HANG Su-qin. Comparative Study of Pig Duodenum Between Tissue Perfusion Culture and Static Culture[J]. Biotechnology Bulletin, 2021, 37(2): 246-252.

Figures/Tables 6

References 28

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