Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (2): 236-245.doi: 10.13560/j.cnki.biotech.bull.1985.2020-0543
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Received:
2020-05-08
Online:
2021-02-26
Published:
2021-02-26
CHEN Zhong, LU Xing-yu. An Orthogonal Method to Study the Thermal Stability of Secondary Structure of Protein[J]. Biotechnology Bulletin, 2021, 37(2): 236-245.
蛋白构象 | 电子跃迁形式 | 负吸收带/nm | 正吸收带/nm |
---|---|---|---|
α-螺旋(α-helix) | n→π* π→π* | 222-208 | 192 |
β-折叠(β-sheet) | n→π* π→π* | 217-218 | 185-200 |
β-转角(β-turn) | n→π* π→π* | 220-230 | 200-205 |
P2结构(poly(L-proline)type II) | π→π* | 190-205 | 220 |
无规卷曲(random coil) | π→π* | 200 | 218 |
蛋白构象 | 电子跃迁形式 | 负吸收带/nm | 正吸收带/nm |
---|---|---|---|
α-螺旋(α-helix) | n→π* π→π* | 222-208 | 192 |
β-折叠(β-sheet) | n→π* π→π* | 217-218 | 185-200 |
β-转角(β-turn) | n→π* π→π* | 220-230 | 200-205 |
P2结构(poly(L-proline)type II) | π→π* | 190-205 | 220 |
无规卷曲(random coil) | π→π* | 200 | 218 |
Wavelength | Fitting Function | Tm(x0,x1) |
---|---|---|
192 nm (极值点) | Ab+((At-Ab)/(1+exp((x0-x)/w)));Ab = 50.036170 +/- 0.175916;At = 14.204700 +/- 0.579455 x0 = 65.962690 +/- 0.370525;w = 9.035970 +/- 0.296864 | 65.962690 +/- 0.370525 |
208 nm (极值点) | Ab+((At-Ab)/(1+exp((x0-x)/w)));Ab = -29.200821 +/- 0.069834;At = -16.976313 +/- 0.200842 x0 = 66.694838 +/- 0.375953;w = 8.872889 +/- 0.315981 | 66.694838 +/- 0.375953 |
222 nm (极值点) | Ab+((At-Ab)/(1+exp((x0-x)/w)));Ab = -28.557619 +/- 0.066161;At = -12.848438 +/- 0.197279 x0 = 65.167471 +/- 0.293993;w = 10.044699 +/- 0.247323 | 65.167471 +/- 0.293993 |
235 nm (任意点) | Ab+((At-Ab)/(1+exp((x0-x)/w)));Ab = -11.088690 +/- 0.012442;At = -6.796280 +/- 0.038061 x0 = 65.810820 +/- 0.203645;w = 8.997813 +/- 0.167840 | 65.810820 +/- 0.203645 |
260 nm (端点) | Ab+((Am-Ab)/(1+exp((x0-x)/w0)))+((At-Am)/(1+exp((x1-x)/w1))) Ab = -2.099029 +/- 0.018708;Am = -2.048087 +/- 0.028690;At = -1.666950 +/- 0.224199 x0 = 54.123624 +/- 1.257872;x1 = 80.424220 +/- 20.694954;w0 = 0.841862 +/- 1.174727 w1 = 17.153602 +/- 7.784341 | 54.123624 +/- 1.257872 80.424220 +/- 20.694954 |
203 nm (交叉点) | a.exp((-(x-x0)^2)/(2.b^2))+ d;a = 1.567304 +/- 0.078462;b = 15.396921 +/- 0.948422 x0 = 52.170676 +/- 0.314500;d = -13.325323 +/- 0.085624 | 52.170676 +/- 0.314500 |
Wavelength | Fitting Function | Tm(x0,x1) |
---|---|---|
192 nm (极值点) | Ab+((At-Ab)/(1+exp((x0-x)/w)));Ab = 50.036170 +/- 0.175916;At = 14.204700 +/- 0.579455 x0 = 65.962690 +/- 0.370525;w = 9.035970 +/- 0.296864 | 65.962690 +/- 0.370525 |
208 nm (极值点) | Ab+((At-Ab)/(1+exp((x0-x)/w)));Ab = -29.200821 +/- 0.069834;At = -16.976313 +/- 0.200842 x0 = 66.694838 +/- 0.375953;w = 8.872889 +/- 0.315981 | 66.694838 +/- 0.375953 |
222 nm (极值点) | Ab+((At-Ab)/(1+exp((x0-x)/w)));Ab = -28.557619 +/- 0.066161;At = -12.848438 +/- 0.197279 x0 = 65.167471 +/- 0.293993;w = 10.044699 +/- 0.247323 | 65.167471 +/- 0.293993 |
235 nm (任意点) | Ab+((At-Ab)/(1+exp((x0-x)/w)));Ab = -11.088690 +/- 0.012442;At = -6.796280 +/- 0.038061 x0 = 65.810820 +/- 0.203645;w = 8.997813 +/- 0.167840 | 65.810820 +/- 0.203645 |
260 nm (端点) | Ab+((Am-Ab)/(1+exp((x0-x)/w0)))+((At-Am)/(1+exp((x1-x)/w1))) Ab = -2.099029 +/- 0.018708;Am = -2.048087 +/- 0.028690;At = -1.666950 +/- 0.224199 x0 = 54.123624 +/- 1.257872;x1 = 80.424220 +/- 20.694954;w0 = 0.841862 +/- 1.174727 w1 = 17.153602 +/- 7.784341 | 54.123624 +/- 1.257872 80.424220 +/- 20.694954 |
203 nm (交叉点) | a.exp((-(x-x0)^2)/(2.b^2))+ d;a = 1.567304 +/- 0.078462;b = 15.396921 +/- 0.948422 x0 = 52.170676 +/- 0.314500;d = -13.325323 +/- 0.085624 | 52.170676 +/- 0.314500 |
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