Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (7): 88-97.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0508

Previous Articles     Next Articles

Identification of Rice Genes Responding to Both the Nematode Effector MgMO237 and Its Interacting Protein OsCRRSP55

LI Zhi-wen1(), LIU Pei-yan1, CHEN Jian-song1,2, LIAO Jin-ling1,2,3, LIN Bo-rong1,2(), ZHUO Kan1,2()   

  1. 1. Laboratory of Plant Nematology,College of Plant Protection,South China Agricultural University,Guangzhou 510642
    2. Guangdong Province Key Laboratory of Microbial Signals and Disease Control,South China Agricultural University,Guangzhou 510642
    3. Guangdong Eco-Engineering Polytechnic,Guangzhou 510520
  • Received:2021-04-18 Online:2021-07-26 Published:2021-08-13
  • Contact: LIN Bo-rong,ZHUO Kan E-mail:lizhiwen2017@126.com;boronglin@scau.edu.cn;zhuokan@scau.edu.cn

Abstract:

Previous studies showed that the Meloidogyne graminicola effector MgMO237 may interact with the rice protein OsCRRSP55 and inhibit rice defense responses against M. graminicola. Plant protein CRRSPs are involved in plant responses to both biotic and abiotic stresses. Understanding the influence of the interaction between MgMO237 and OsCRRSP55 on the expressions of their downstream genes will provide a theoretical basis for elucidating the mechanism of rice defense responses against root-knot nematodes. Bioinformatics analysis indicated that the OsCRRSP55 protein contained an N-terminal signal peptide and two conserved domains called the domain unknown function 26(DUF26),in which occurred a conserved motif C-X8-C-X2-C,but had no transmembrane domains. RT-qPCR analysis indicated that the OsCRRSP55 expressed in the rice roots and it was significantly up-regulated in the galls at 7 d infection by M. graminicola compared with that in healthy root tip. The expression levels of OsCRRSP55 and its promoter binding transcription factor OsWRKY47 significantly increased in the rice treated with salicylic acid and methyl jasmonate compared with those in wild-type ones. Transcriptome sequencing showed that a jasmonic acid-responsive gene OsERF87 was down-regulated in MgMO237-transgenic rice,but up-regulated in rice overexpressing OsCRRSP55 by RT-qPCR,showing the OsERF87 was co-regulated by OsCRRSP55 and MgMO237. In sum,these results demonstrate that the M. graminicola effector MgMO237 may affect the jasmonic acid signal transmission by interacting with OsCRRSP55,which results in the suppression of plant defense responses to M. graminicola.

Key words: Meloidogyne graminicola, MgMO237, OsCRRSP55, OsERF87, jasmonate signaling pathway