Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (8): 131-140.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0191

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Sequencing and Analysis of Full-length Transcriptome from Medicago truncatula

SHANG Xiao-yao(), ZHOU Ling-fang, YIN Qian-qian, CHAO Yue-hui()   

  1. School of Grassland Science,Beijing Forestry University,Beijing 100083
  • Received:2021-02-18 Online:2021-08-26 Published:2021-09-10
  • Contact: CHAO Yue-hui E-mail:sxy997987281@163.com;chaoyuehui@bjfu.edu.cn

Abstract:

For deep analysis and exploring of the complete structure of mRNA from Medicago truncatula,a model plant in Leguminosae,the single-molecule long-read sequencing technology(SMRT)was used to sequence and analyze the full-length transcriptome of M. truncatula. A total of 7 728 183 subreads and 509 014 full-length non-chimeric reads(FLNC)were obtained. By comparing the reference genomes of R108 and A17,94.36% and 93.01% isoforms were identified as mapped reads,respectively. A total of 8 406 alternative splicing events was identified and the majority was intron retention(RI). The 23 926 genes were detected,of 12 049 genes had 295 545 transcripts,in which at least there was one poly(A)site. In addition,3 223 transcription factors,6 595 long non-coding RNAs(lncRNA)and 479 fusion transcripts were identified. The results showed the feasibility of deep sequencing M. truncatula transcriptome by SMRT,which provides data supplement for better utilizing the genome resource of M. truncatula.

Key words: Medicago truncatula, single-molecule long-read sequencing, alternative splice, fusion transcript