Biotechnology Bulletin ›› 2021, Vol. 37 ›› Issue (10): 137-142.doi: 10.13560/j.cnki.biotech.bull.1985.2020-1509
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FU Qiang(), GUO Yan-ting, CHEN Jun-zhen, WANG Jin-quan, SHI Hui-jun()
Received:
2020-12-13
Online:
2021-10-26
Published:
2021-11-12
Contact:
SHI Hui-jun
E-mail:466183013@qq.com;shihuijunmm@163.com
FU Qiang, GUO Yan-ting, CHEN Jun-zhen, WANG Jin-quan, SHI Hui-jun. Preparation and Identification of Polyclonal Antibody Against Ion Channel Protein p7 Polypeptide of Bovine Viral Diarrhea Virus[J]. Biotechnology Bulletin, 2021, 37(10): 137-142.
血清稀释比例Serum dilution ratio | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
10 | 100 | 1000 | 1E+04 | 1E+05 | 1E+06 | 1E+07 | 1E+08 | 1E+09 | 1E+10 | 1E+11 | 1E+12 | |
纯化后抗体Purified antibody | 2.24 | 2.254 | 1.175 | 0.437 | 0.368 | 0.288 | 0.269 | 0.262 | 0.279 | 0.288 | 0.237 | 0.161 |
2.141 | 2.186 | 1.01 | 0.456 | 0.446 | 0.305 | 0.31 | 0.288 | 0.303 | 0.286 | 0.271 | 0.244 | |
PBS对照PBS Control | 0.176 | 0.186 | 0.213 | 0.148 | 0.113 | 0.133 | 0.111 | 0.111 | 0.104 | 0.14 | 0.063 | 0.102 |
0.148 | 0.139 | 0.12 | 0.107 | 0.088 | 0.07 | 0.076 | 0.075 | 0.07 | 0.053 | 0.044 | 0.055 | |
免疫前Pre immunization | 0.139 | 0.132 | 0.126 | 0.126 | 0.19 | 0.111 | 0.163 | 0.164 | 0.178 | 0.124 | 0.189 | 0.156 |
Table 1 Detection of antibody titer using indirect ELISA
血清稀释比例Serum dilution ratio | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
10 | 100 | 1000 | 1E+04 | 1E+05 | 1E+06 | 1E+07 | 1E+08 | 1E+09 | 1E+10 | 1E+11 | 1E+12 | |
纯化后抗体Purified antibody | 2.24 | 2.254 | 1.175 | 0.437 | 0.368 | 0.288 | 0.269 | 0.262 | 0.279 | 0.288 | 0.237 | 0.161 |
2.141 | 2.186 | 1.01 | 0.456 | 0.446 | 0.305 | 0.31 | 0.288 | 0.303 | 0.286 | 0.271 | 0.244 | |
PBS对照PBS Control | 0.176 | 0.186 | 0.213 | 0.148 | 0.113 | 0.133 | 0.111 | 0.111 | 0.104 | 0.14 | 0.063 | 0.102 |
0.148 | 0.139 | 0.12 | 0.107 | 0.088 | 0.07 | 0.076 | 0.075 | 0.07 | 0.053 | 0.044 | 0.055 | |
免疫前Pre immunization | 0.139 | 0.132 | 0.126 | 0.126 | 0.19 | 0.111 | 0.163 | 0.164 | 0.178 | 0.124 | 0.189 | 0.156 |
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