Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (1): 179-186.doi: 10.13560/j.cnki.biotech.bull.1985.2021-0289
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WU Kun-kun1,2(), XU Xing1,2, JI Ce1,2, REN Jian-feng1,2, LI Wei-ming3, ZHANG Qing-hua1,2()
Received:
2021-03-10
Online:
2022-01-26
Published:
2022-02-22
Contact:
ZHANG Qing-hua
E-mail:1017676610@qq.com;qhzhang@shou.edu.cn
WU Kun-kun, XU Xing, JI Ce, REN Jian-feng, LI Wei-ming, ZHANG Qing-hua. Eukaryotic Expression Vector Construction of Danio rerio notch3 Gene and Its Expression Analysis[J]. Biotechnology Bulletin, 2022, 38(1): 179-186.
长度 Length/bp | 引物序列 Sequence of primer | 酶切位点选择 Selection of enzyme cutting sites |
---|---|---|
2 487 | TGGGCTAGGCTACTGCTAGTAGG | Pst I和BamH I |
TCAAGCAAACACCTGCATCTTC |
Table 1 N3ICD primer amplification sequence
长度 Length/bp | 引物序列 Sequence of primer | 酶切位点选择 Selection of enzyme cutting sites |
---|---|---|
2 487 | TGGGCTAGGCTACTGCTAGTAGG | Pst I和BamH I |
TCAAGCAAACACCTGCATCTTC |
Fig. 2 Cloning of notch3 gene Intracellular domain(A)and construction of recombinant plasmid(B) M:Molecular weight standard. 1:Intracellular segment of notch3 gene. 2, 3:Positive clone
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