Endophytes can provide nutrients for plants and also promote plant growth through metabolites. Currently,there are few studies on endophytes in Passiflora edulia Sims. P. edulia Sims is a herbaceous vine of the Passiflora genus of the Passiflora family and mainly distributed in tropical and subtropical regions. By isolating and purifying the endophytes of P. edulia Sims,the obtained strains were clustered according to the IS-PCR results,the 16S rRNA genes of the representative strains were sequenced and phylogenetic analysis was performed,and their nitrogenase activity,auxin-producing capacity,phosphorus-dissolving ability,potassium-dissolving ability,protease production ability and physiological and biochemical characteristics were tested. The isolated and purified 51 strains of P. edulia Sims endophytic bacteria,clustered into 12 groups,belonging to Bacillus altitudinis,Bacillus circulans,Lysinibacillus macrolides,Brevibacillus antibioticus,Paenibacillus illinoisensis,Microbacterium zeae,Rhizobium pusense,Beijerinckia fluminensis,Achromobacter mucicolens,Stenotrophomonas maltophilia,Klebsiella michiganensis and Klebsiella pneumonia,the growth-promoting characteristics of the isolated endophytes were determined,and the results showed that the isolated representative endophytes had abundant functions,10 of them had the ability of secreting auxin,5 of them had nitrogen-fixing function,6 of them had phosphorus-dissolving function,7 of them had potassium-dissolving ability,3 of them had the ability of secreting siderophores,and 5 of them had the ability of producing protease. This experiment shows that the endophytes isolated from P. edulia Sims have both population diversity and functional diversity. These strains with different characteristics are expected to be utilized in agricultural production to exert their values.
