Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (11): 258-268.doi: 10.13560/j.cnki.biotech.bull.1985.2021-1617
Previous Articles Next Articles
WANG Xiao-tao(), ZOU Hang, WU Yi, XIANG Shen-wei, LV Hua, LIU Chao-lan, LIN Jia-fu, WANG Xin-rong, CHU Yi-wen, SONG Tao()
Received:
2021-12-30
Online:
2022-11-26
Published:
2022-12-01
Contact:
SONG Tao
E-mail:wangxiaotao@stu.cdu.edu.cn;songtao@cdu.edu.cn
WANG Xiao-tao, ZOU Hang, WU Yi, XIANG Shen-wei, LV Hua, LIU Chao-lan, LIN Jia-fu, WANG Xin-rong, CHU Yi-wen, SONG Tao. Heterologous Expression and Enzymatic Properties Analysis of Novel β-agarase Aga2 from Paraglaciecola hydrolytica[J]. Biotechnology Bulletin, 2022, 38(11): 258-268.
Fig.4 Effects of IPTG,temperature and induction time concentration on Aga2 M:Protein marker. A:1-7:protein samples when the concentration of IPTG is 0,30,60,90,120,150和 180 μmol/L,respectively. C:1-5:protein samples when the induction temperatures is 16℃,20℃,24℃,28℃,and 32℃. E:1-6:protein samples when the induction times is 0,3,6,9,12 and 15 h
Fig.12 CNMR analysis of agar hydrolysis product catalyz-ed by Aga2 G and A:D-galactose and 3,6-Anhydro-L-galactose;r and nr:reducing and non-reducing ends;α and β:α carbon atoms and β carbon atoms
Fig. 13 TLC analysis of hydrolysis product by Aga2 during different time points NA8:Neoagarooctaose standard;NA6:neoagarohexaose standard;NA4:neoagarotetraose standard;NA2:neoagarobiose standard
Fig. 15 TLC analysis of different oligosaccharide hydroly-sis product catalyzed by Aga2 1-4:Mixed reaction products of Aga2 and neoagarobiose,Aga2 and neoagarote-traose,Aga2 and neoagarohexaose,Aga2 and neoagarooctaose,respectively. 5-8:Neoagarobiose standard,neoagarotetraose standard,neoagarohexaose standard,and neoagarooctaose standard,respectively
[1] |
Liu N, Yang M, Mao XZ, et al. Molecular cloning and expression of a new α-neoagarobiose hydrolase from Agarivorans gilvus WH0801 and enzymatic production of 3, 6-anhydro-l-galactose[J]. Biotechnol Appl Biochem, 2016, 63(2):230-237.
doi: 10.1002/bab.1363 URL |
[2] |
Kobayashi R, Takisada M, Suzuki T, et al. Neoagarobiose as a novel moisturizer with whitening effect[J]. Biosci Biotechnol Biochem, 1997, 61(1):162-163.
doi: 10.1271/bbb.61.162 URL |
[3] | 李海新, 梁家铭, 李蓉, 等. 琼胶寡糖的抗氧化活性研究[J]. 广州化工, 2018, 46(20):66-68. |
Li HX, Liang JM, Li R, et al. Study on antioxidant activity of agar-oligosaccharide[J]. Guangzhou Chem Ind, 2018, 46(20):66-68. | |
[4] |
Ariga O, Okamoto N, Harimoto N, et al. Purification and characterization of α-neoagarooligosaccharide hydrolase from Cellvibrio sp. OA-2007[J]. J Microbiol Biotechnol, 2014, 24(1):48-51.
doi: 10.4014/jmb.1307.07018 URL |
[5] |
Jin M, Liu HF, Hou YP, et al. Preparation, characterization and alcoholic liver injury protective effects of algal oligosaccharides from Gracilaria lemaneiformis[J]. Food Res Int, 2017, 100(Pt 2):186-195.
doi: 10.1016/j.foodres.2017.08.032 URL |
[6] |
Higashimura Y, Naito Y, Takagi T, et al. Protective effect of agaro-oligosaccharides on gut dysbiosis and colon tumorigenesis in high-fat diet-fed mice[J]. Am J Physiol Gastrointest Liver Physiol, 2016, 310(6):G367-G375.
doi: 10.1152/ajpgi.00324.2015 URL |
[7] |
Liang SS, Chen YP, Chen YH, et al. Characterization and overexpression of a novel β-agarase from Thalassomonas Agarivorans[J]. J Appl Microbiol, 2014, 116(3):563-572.
doi: 10.1111/jam.12389 pmid: 24206167 |
[8] |
Zhang WB, Xu JN, Liu D, et al. Characterization of an α-agarase from Thalassomonas sp. LD5 and its hydrolysate[J]. Appl Microbiol Biotechnol, 2018, 102(5):2203-2212.
doi: 10.1007/s00253-018-8762-6 URL |
[9] |
Fu XT, Kim SM. Agarase:review of major sources, categories, purification method, enzyme characteristics and applications[J]. Mar Drugs, 2010, 8(1):200-218.
doi: 10.3390/md8010200 URL |
[10] |
Jahromi ST, Barzkar N. Future direction in marine bacterial agarases for industrial applications[J]. Appl Microbiol Biotechnol, 2018, 102(16):6847-6863.
doi: 10.1007/s00253-018-9156-5 pmid: 29909571 |
[11] |
Ohta Y, Hatada Y, Nogi Y, et al. Enzymatic properties and nucleotide and amino acid sequences of a thermostable β-agarase from a novel species of deep-sea Microbulbifer[J]. Appl Microbiol Biotechnol, 2004, 64(4):505-514.
pmid: 15088129 |
[12] |
Hsu PH, Wei CH, Lu WJ, et al. Extracellular production of a novel endo-β-agarase AgaA from Pseudomonas vesicularis MA103 that cleaves agarose into neoagarotetraose and neoagarohexaose[J]. Int J Mol Sci, 2015, 16(3):5590-5603.
doi: 10.3390/ijms16035590 URL |
[13] |
Chen XL, Hou YP, Jin M, et al. Expression and characterization of a novel thermostable and pH-stable β-agarase from deep-sea bacterium Flammeovirga sp. OC4[J]. J Agric Food Chem, 2016, 64(38):7251-7258.
doi: 10.1021/acs.jafc.6b02998 URL |
[14] |
Park DY, Chi WJ, Park JS, et al. Cloning, expression, and biochemical characterization of a GH16 β-agarase AgaH71 from Pseudoalteromonas hodoensis H7[J]. Appl Biochem Biotechnol, 2015, 175(2):733-747.
doi: 10.1007/s12010-014-1294-3 URL |
[15] |
Kim JH, Yun EJ, Seo N, et al. Enzymatic liquefaction of agarose above the Sol-gel transition temperature using a thermostable endo-type β-agarase, Aga16B[J]. Appl Microbiol Biotechnol, 2017, 101(3):1111-1120.
doi: 10.1007/s00253-016-7831-y pmid: 27664160 |
[16] |
Krogh A, Larsson B, von Heijne G, et al. Predicting transmembrane protein topology with a hidden Markov model:application to complete genomes[J]. J Mol Biol, 2001, 305(3):567-580.
doi: 10.1006/jmbi.2000.4315 pmid: 11152613 |
[17] |
Petersen TN, Brunak S, von Heijne G, et al. SignalP 4. 0:discriminating signal peptides from transmembrane regions[J]. Nat Methods, 2011, 8(10):785-786.
doi: 10.1038/nmeth.1701 pmid: 21959131 |
[18] |
Chou KC, Shen HB. Cell-PLoc:a package of Web servers for predicting subcellular localization of proteins in various organisms[J]. Nat Protoc, 2008, 3(2):153-162.
doi: 10.1038/nprot.2007.494 URL |
[19] | Marchler-Bauer A, Derbyshire MK, Gonzales NR, et al. CDD:NCBI’s conserved domain database[J]. Nucleic Acids Res, 2015, 43(Database issue):D222-D226. |
[20] |
Song T, Xu H, Wei CC, et al. Horizontal transfer of a novel soil agarase gene from marine bacteria to soil bacteria via human microbiota[J]. Sci Rep, 2016, 6:34103.
doi: 10.1038/srep34103 pmid: 27756908 |
[21] | 许振兴. 四株海洋新菌的鉴定及菌株HQM9T和Q1T的琼胶降解酶研究[D]. 济南: 山东大学, 2017. |
Xu ZX. Polyphasic taxonomy analysis of four novel marine bacterial strains and studies on agar-degrading eneymes produced by strains HQM9T and Q1T[D]. Jinan: Shandong University, 2017. | |
[22] |
Lee Y, Oh C, de Zoysa M, et al. Molecular cloning, overexpression, and enzymatic characterization of glycosyl hydrolase family 16 β-Agarase from marine bacterium Saccharophagus sp. AG21 in Escherichia coli[J]. J Microbiol Biotechnol, 2013, 23(7):913-922.
doi: 10.4014/jmb.1209.09009 URL |
[23] |
Lee DG, Jeon MJ, Lee SH. Cloning, expression, and characterization of a glycoside hydrolase family 118 beta-agarase from Agarivorans sp. JA-1[J]. J Microbiol Biotechnol, 2012, 22(12):1692-1697.
doi: 10.4014/jmb.1209.09033 URL |
[24] | 金佳, 江承程, 毛相朝. α-琼胶酶OUC-GaJJ96的异源表达及酶学性质[J]. 食品科学技术学报, 2020, 38(6):47-54. |
Jin J, Jiang CC, Mao XZ. Heterologous expression and enzymatic properties of α-agarase OUC-GaJJ96[J]. J Food Sci Technol, 2020, 38(6):47-54.
doi: 10.1046/j.1365-2621.2003.00630.x URL |
|
[25] |
Cui X, Jiang YC, Chang LY, et al. Heterologous expression of an agarase gene in Bacillus subtilis, and characterization of the agarase[J]. Int J Biol Macromol, 2018, 120(Pt A):657-664.
doi: 10.1016/j.ijbiomac.2018.07.118 URL |
[26] |
Kim HT, Lee S, Lee D, et al. Overexpression and molecular characterization of Aga50D from Saccharophagus degradans 2-40:an exo-type beta-agarase producing neoagarobiose[J]. Appl Microbiol Biotechnol, 2010, 86(1):227-234.
doi: 10.1007/s00253-009-2256-5 URL |
[27] |
Liu J, Liu Z, Jiang CC, et al. Biochemical characterization and substrate degradation mode of a novel α-agarase from Catenovulum agarivorans[J]. J Agric Food Chem, 2019, 67(37):10373-10379.
doi: 10.1021/acs.jafc.9b03073 URL |
[28] |
Jung S, Jeong BC, Hong SK, et al. Cloning, expression, and biochemical characterization of a novel acidic GH16 β-agarase, AgaJ11, from Gayadomonas joobiniege G7[J]. Appl Biochem Biotechnol, 2017, 181(3):961-971.
doi: 10.1007/s12010-016-2262-x URL |
[29] | Aziz GM, Ali HM. Purification and characterization of agarase from Bacillus sp. H12[J]. Curr Res J Biol Sci, 2013, 5(1):13-18. |
[30] |
Lee CH, Lee CR, Hong SK. Biochemical characterization of a novel cold-adapted agarotetraose-producing α-agarase, AgaWS5, from Catenovulum sediminis WS1-A[J]. Appl Microbiol Biotechnol, 2019, 103(20):8403-8411.
doi: 10.1007/s00253-019-10056-1 URL |
[31] |
Fu XT, Lin H, Kim SM. Purification and characterization of a novel β-agarase, AgaA34, from Agarivorans albus YKW-34[J]. Appl Microbiol Biotechnol, 2008, 78(2):265-273.
doi: 10.1007/s00253-007-1303-3 URL |
[32] |
Hou YP, Chen XL, Chan ZH, et al. Expression and characterization of a thermostable and pH-stable β-agarase encoded by a new gene from Flammeovirga pacifica WPAGA1[J]. Process Biochem, 2015, 50(7):1068-1075.
doi: 10.1016/j.procbio.2015.04.005 URL |
[1] | WANG Shuai, FENG Yu-mei, BAI Miao, DU Wei-jun, YUE Ai-qin. Functional Analysis of Soybean Gene GmHMGR Responding to Exogenous Hormones and Abiotic Stresses [J]. Biotechnology Bulletin, 2023, 39(7): 131-142. |
[2] | MA Yu-qian, SUN Dong-hui, YUE Hao-feng, XIN Jia-yu, LIU Ning, CAO Zhi-yan. Identification, Heterologous Expression and Functional Analysis of a GH61 Family Glycoside Hydrolase from Setosphaeria turcica with the Assisting Function in Degrading Cellulose [J]. Biotechnology Bulletin, 2023, 39(4): 124-135. |
[3] | CHEN Nan-nan, WANG Chun-lai, JIANG Zhen-zhong, JIAO Peng, GUAN Shu-yan, MA Yi-yong. Genetic Transformation and Chilling Resistance Analysis of Maize ZmDHN15 Gene in Tobacco [J]. Biotechnology Bulletin, 2023, 39(4): 259-267. |
[4] | YANG Jun-zhao, ZHANG Xin-rui, ZHAO Guo-zhu, ZHENG Fei. Structure and Function Analysis of Novel GH5 Multi-domain Cellulase [J]. Biotechnology Bulletin, 2023, 39(4): 71-80. |
[5] | WANG Yu-chen, DING Zun-dan, GUAN Fei-fei, TIAN Jian, LIU Guo-an, WU Ning-feng. Identification of the Thermostable Laccase Gene ba4 and Characterization of Its Enzymatic Properties [J]. Biotechnology Bulletin, 2022, 38(8): 252-260. |
[6] | FU Qiao, LIN Qi-lan, XUE Qiang, XIONG Hai-rong, WANG Ya-wei. Effects of CBM41 N-terminal Truncation on the Enzymological Properties of the Pullulanase from Bacillus subtilis 168 [J]. Biotechnology Bulletin, 2022, 38(6): 245-251. |
[7] | NIU Xin, ZHANG Ying, WANG Mao-jun, LIU Wen-long, LU Fu-ping, LI Yu. Effects of Different Integration Sites on the Expression of Exogenous Alkaline Protease in Bacillus amyloliquefaciens [J]. Biotechnology Bulletin, 2022, 38(4): 253-260. |
[8] | WANG Bo-ya, JIANG Yong, HUANG Yan, CAO Ying, HU Shang-lian. Cloning and Functional Analysis of BeCesA4 in Bambusa emeiensis [J]. Biotechnology Bulletin, 2022, 38(11): 185-193. |
[9] | ZHANG Tong-tong, ZHENG Deng-yu, WU Zhong-yi, ZHANG Zhong-bao, YU Rong. Functional Analysis of ZmNF-YB13 Responding to Drought and Salt Stress [J]. Biotechnology Bulletin, 2022, 38(10): 115-123. |
[10] | LIAO Zhao-min, CAI Jun, LIN Jian-guo, DU Xin, WANG Chang-gao. Expression of Glucose Oxidase Gene from Aspergillus niger in Pichia pastoris and Optimization of Enzyme Production Conditions [J]. Biotechnology Bulletin, 2021, 37(6): 97-107. |
[11] | ZHANG Yao-xin, WANG Liang-jie, ZHENG Wen, XU Han-qin, ZHENG Lian, ZHONG Jing. Study on Enzyme Production of a Chitinase-producing Strain Achromobacter sp. ZWW8 by Fermentation and Its Enzymatic Characterization [J]. Biotechnology Bulletin, 2021, 37(4): 96-106. |
[12] | LIU Shan, YE Wei, ZHU Mu-zi, LI Sai-ni, DENG Zhang-shuang, ZHANG Wei-min. Cloning,Expression and Characterization of a Novel Acyltransferase GPAT [J]. Biotechnology Bulletin, 2021, 37(11): 257-266. |
[13] | ZHAO Hai-yan, SONG Chen-bin, LIU Zheng-ya, MA Xing-rong, SHANG Hui-hui, LI An-hua, GUAN Xian-jun, WANG Jian-she. Cloning,Recombinant Expression and Enzymatic Properties of α-Amylase Gene from Laceyella sp. [J]. Biotechnology Bulletin, 2020, 36(8): 23-33. |
[14] | WANG Hui-lan, WU Jin-yong, CHEN Xiang-song, YUAN Li-xia, ZHU Wei-wei, YAO Jian-ming. Immobilization of N-acetylneuraminic Acid Aldolaseand Properties of the Immobilized Enzyme [J]. Biotechnology Bulletin, 2020, 36(6): 165-173. |
[15] | LI Wei-na, SHEN Dong-ling, ZHANG Yu-xing, LIU Xue-tong, IRBIS Chagan. Cloning and Enzymatic Identification of Thermo-tolerant and Endotype Alginate Lyase Gene from Mangrovibacterium sp. SH-52 [J]. Biotechnology Bulletin, 2020, 36(12): 82-90. |
Viewed | ||||||
Full text |
|
|||||
Abstract |
|
|||||