Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (11): 277-285.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0043
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CAO Hai-peng1,2(), ZHANG Shu-meng1,2, DIAO Jing3,4, XU La3,4, GAI Chun-lei3,4()
Received:
2022-01-10
Online:
2022-11-26
Published:
2022-12-01
Contact:
GAI Chun-lei
E-mail:hpcao@shou.edu.cn;chunlei317@163.com
CAO Hai-peng, ZHANG Shu-meng, DIAO Jing, XU La, GAI Chun-lei. Isolation,Identification and Characterization of Rhodobacter azotoformans SY5 with Enhancing Effect on the Disease Resistance of Eriocheir sinensis[J]. Biotechnology Bulletin, 2022, 38(11): 277-285.
Fig.2 Phylogenetic tree based on 16S rRNA sequences of isolate SY5 The sequence number in the bracket refers to the GenBank accession number of the strain;numbers at the branch points refer to the bootstrap values on Neighbor-joining analysis of 1 000 replications data sets;the scale bar 0.02 indicates sequence divergence
项目Test | 结果Result | 项目Test | 结果Result | ||
---|---|---|---|---|---|
SY5 | 固氮红细菌R. azotoformans[ | SY5 | 固氮红细菌R. azotoformans[ | ||
甲酸钠Sodium formate | + | + | 半乳糖醇 Dulcitol | + | + |
乙酸钠Sodium acetate | + | + | 甘露醇Mannitol | + | + |
丙酸钠Sodium propionate | - | - | 柠檬酸钠Trisodium citrate dihydrate | + | + |
甘油Glycerol | + | + | 酒石酸钠Sodium tartaric | - | - |
葡萄糖Glucose | + | + | 葡萄糖酸钠Sodium citrate | + | + |
果糖Fructose | + | + | 乙醇Ethanol | + | + |
过氧化氢酶Catalase | + | + | 硫代硫酸钠Sodium thiosulfate | - | - |
甘露糖Mannose | + | + | 硫化钠Sodium sulfide | - | - |
苯甲酸钠Sodium benzoic | - | - | 3%NaCl生长 3%NaCl growth | + | + |
Table 1 Physiological and biochemical test of isolate SY5
项目Test | 结果Result | 项目Test | 结果Result | ||
---|---|---|---|---|---|
SY5 | 固氮红细菌R. azotoformans[ | SY5 | 固氮红细菌R. azotoformans[ | ||
甲酸钠Sodium formate | + | + | 半乳糖醇 Dulcitol | + | + |
乙酸钠Sodium acetate | + | + | 甘露醇Mannitol | + | + |
丙酸钠Sodium propionate | - | - | 柠檬酸钠Trisodium citrate dihydrate | + | + |
甘油Glycerol | + | + | 酒石酸钠Sodium tartaric | - | - |
葡萄糖Glucose | + | + | 葡萄糖酸钠Sodium citrate | + | + |
果糖Fructose | + | + | 乙醇Ethanol | + | + |
过氧化氢酶Catalase | + | + | 硫代硫酸钠Sodium thiosulfate | - | - |
甘露糖Mannose | + | + | 硫化钠Sodium sulfide | - | - |
苯甲酸钠Sodium benzoic | - | - | 3%NaCl生长 3%NaCl growth | + | + |
抗生素 Antibiotics | 抑菌圈直径判断标准 Assessment standard of inhibition zone diameter /mm | 药物含量 Content /(μg·disc-1) | 抑菌圈直径 Diameter of inhibition zone/mm | 敏感性 Susceptibility | ||
---|---|---|---|---|---|---|
不敏感R Resistance | 中度敏感I Intermediate sensitivity | 高度敏感S Sensitivity | ||||
阿莫西林Amoxicillin | ≤13 | 14-17 | ≥18 | 10 | 24.30±0.78 | S |
新霉素Neomycin | ≤12 | 13-16 | ≥17 | 30 | 24.05±0.83 | S |
庆大霉素Gentamicin | ≤12 | 13-14 | ≥15 | 10 | 18.20±0.44 | S |
四环素Tetracycline | ≤14 | 15-18 | ≥19 | 30 | 19.22±0.56 | S |
多黏菌素B Polymyxin B | ≤8 | 9-11 | ≥12 | 30 | 15.21±0.82 | S |
恩诺沙星Enrofloxacin | ≤15 | 16-18 | ≥19 | 5 | 26.55±0.70 | S |
环丙沙星Ciprofloxacin | ≤15 | 16-20 | ≥21 | 5 | 24.20±0.65 | S |
卡那霉素Kanamycin | ≤13 | 14-17 | ≥18 | 30 | 24.40±0.45 | S |
氨苄西林Ampicillin | ≤13 | 14-16 | ≥17 | 10 | 22.50±0.25 | S |
罗红霉素Roxithromycin | ≤10 | 11-15 | ≥16 | 15 | 16.50±1.06 | S |
磷霉素Fosfomycin | ≤12 | 13-15 | ≥16 | 200 | 23.12±0.60 | S |
链霉素Streptomycin | ≤11 | 12-14 | ≥15 | 10 | 25.25±0.85 | S |
多西环素Doxycycline | ≤17 | 18-22 | ≥23 | 30 | 20.85±0.65 | I |
Table 2 Antibiotic susceptibility of isolate SY5
抗生素 Antibiotics | 抑菌圈直径判断标准 Assessment standard of inhibition zone diameter /mm | 药物含量 Content /(μg·disc-1) | 抑菌圈直径 Diameter of inhibition zone/mm | 敏感性 Susceptibility | ||
---|---|---|---|---|---|---|
不敏感R Resistance | 中度敏感I Intermediate sensitivity | 高度敏感S Sensitivity | ||||
阿莫西林Amoxicillin | ≤13 | 14-17 | ≥18 | 10 | 24.30±0.78 | S |
新霉素Neomycin | ≤12 | 13-16 | ≥17 | 30 | 24.05±0.83 | S |
庆大霉素Gentamicin | ≤12 | 13-14 | ≥15 | 10 | 18.20±0.44 | S |
四环素Tetracycline | ≤14 | 15-18 | ≥19 | 30 | 19.22±0.56 | S |
多黏菌素B Polymyxin B | ≤8 | 9-11 | ≥12 | 30 | 15.21±0.82 | S |
恩诺沙星Enrofloxacin | ≤15 | 16-18 | ≥19 | 5 | 26.55±0.70 | S |
环丙沙星Ciprofloxacin | ≤15 | 16-20 | ≥21 | 5 | 24.20±0.65 | S |
卡那霉素Kanamycin | ≤13 | 14-17 | ≥18 | 30 | 24.40±0.45 | S |
氨苄西林Ampicillin | ≤13 | 14-16 | ≥17 | 10 | 22.50±0.25 | S |
罗红霉素Roxithromycin | ≤10 | 11-15 | ≥16 | 15 | 16.50±1.06 | S |
磷霉素Fosfomycin | ≤12 | 13-15 | ≥16 | 200 | 23.12±0.60 | S |
链霉素Streptomycin | ≤11 | 12-14 | ≥15 | 10 | 25.25±0.85 | S |
多西环素Doxycycline | ≤17 | 18-22 | ≥23 | 30 | 20.85±0.65 | I |
浓度 Concentration/(CFU · g-1) | 蟹数 Crab No. /ind. | 累计死亡数Total number of deaths /ind. | 平均死亡率 Average mortality/% | ||||||
---|---|---|---|---|---|---|---|---|---|
1 d | 2 d | 3 d | 4 d | 5 d | 6 d | 7 d | |||
0 | 10 | 0 | 3 | 4 | 6 | 6 | 8 | 9 | 83.33±11.55 |
10 | 0 | 2 | 3 | 3 | 5 | 7 | 9 | ||
10 | 0 | 1 | 2 | 3 | 5 | 5 | 7 | ||
6.0×105 | 10 | 0 | 1 | 1 | 1 | 2 | 2 | 2 | 33.33±11.55 |
10 | 0 | 1 | 1 | 1 | 2 | 3 | 4 | ||
10 | 0 | 0 | 1 | 1 | 1 | 2 | 4 | ||
6.0×106 | 10 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 13.33±5.77 |
10 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | ||
10 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | ||
6.0×107 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 6.66±5.77 |
10 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | ||
10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
6.0×108 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0±0 |
10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
6.0×109 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0±0 |
10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Table 3 Mortality of E. sinensis artificially infected with C. freundii at different final concentrations of isolate SY5
浓度 Concentration/(CFU · g-1) | 蟹数 Crab No. /ind. | 累计死亡数Total number of deaths /ind. | 平均死亡率 Average mortality/% | ||||||
---|---|---|---|---|---|---|---|---|---|
1 d | 2 d | 3 d | 4 d | 5 d | 6 d | 7 d | |||
0 | 10 | 0 | 3 | 4 | 6 | 6 | 8 | 9 | 83.33±11.55 |
10 | 0 | 2 | 3 | 3 | 5 | 7 | 9 | ||
10 | 0 | 1 | 2 | 3 | 5 | 5 | 7 | ||
6.0×105 | 10 | 0 | 1 | 1 | 1 | 2 | 2 | 2 | 33.33±11.55 |
10 | 0 | 1 | 1 | 1 | 2 | 3 | 4 | ||
10 | 0 | 0 | 1 | 1 | 1 | 2 | 4 | ||
6.0×106 | 10 | 0 | 0 | 0 | 1 | 1 | 1 | 2 | 13.33±5.77 |
10 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | ||
10 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | ||
6.0×107 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 6.66±5.77 |
10 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | ||
10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
6.0×108 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0±0 |
10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
6.0×109 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0±0 |
10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
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