Biotechnology Bulletin ›› 2022, Vol. 38 ›› Issue (11): 277-285.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0043

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Isolation,Identification and Characterization of Rhodobacter azotoformans SY5 with Enhancing Effect on the Disease Resistance of Eriocheir sinensis

CAO Hai-peng1,2(), ZHANG Shu-meng1,2, DIAO Jing3,4, XU La3,4, GAI Chun-lei3,4()   

  1. 1. National Pathogen Collection Center for Aquatic Animals,Shanghai Ocean University,Shanghai 201306
    2. Shanghai Engineering Research Center for Aquaculture,Shanghai 201306
    3. Shandong Key Laboratory of Disease Control in Mariculture,Qingdao 266104
    4. Marine Science Research Institute of Shandong Province,Qingdao 266104
  • Received:2022-01-10 Online:2022-11-26 Published:2022-12-01
  • Contact: GAI Chun-lei E-mail:hpcao@shou.edu.cn;chunlei317@163.com

Abstract:

In order to enrich microbial resources with the potential application in the prevention and control of diseases in Eriocheir sinensis, strain SY5 of aerobic photosynthetic bacterium was isolated from the sediment of culture pond with carotenoid production ability as the evaluation index, was identified based on morphological, biochemical and molecular identification, and its antibiotic susceptibility and protective effect on E. sinensis against Citrobacter freundii infection were further evaluated. The results indicated that the SY5 isolate was identified phenotypically and molecularly as Rhodobacter azotoformans. It was Gram negative, formed colonies with the characteristics of dark red, round, smooth, moist and regular-edged on nutrient agar plate, showed the closest genetic relationship with R. azotoformans YLK20, and demonstrated highly sensitive to amoxicillin, neomycin, gentamicin, tetracycline, polymyxin B, enrofloxacin, ciprofloxacin, kanamycin, ampicillin, roxithromycin, fosfomycin, streptomycin and moderately sensitive to doxycycline. Besides, it demonstrated effective protection rates of 60.0% to 100% at final doses of 6.0×105 to 6.0×109 CFU/g diets against C. freundi infection in E. sinensis. These data lays a foundation for the later development of R. azotoformans SY5 as a feed additive for E. sinensis aquaculture.

Key words: Rhodobacter azotoformans, Eriocheir sinensis, characterization, Citrobacter freundii