Comparison of Methods for Rapid Determination of Cotton Ploidy by Flow Cytometry

doi:10.13560/j.cnki.biotech.bull.1985.2022-0783

Abstract

Abstract:

Rapid detection of cotton DNA ploidy by flow cytometry provides basic data for cotton ploidy identification and breeding research. The old and young leaves of Gossypium hirsutum Linn. Coker 312 were used as test material，and cell nuclei were extracted using WPB and LB01 dissociation solution and stained with PI for the ploidy detection by flow cytometry. The result showed that nuclei extracted from the cotton young leaves using WPB dissociation solution were more effective for ploidy detection，which had clear and focused peak particle，little background debris and obtained clear peak，less miscellaneous peak，less cellular debris and more nuclei. In conclusion，a method for rapid detection of cotton DNA ploidy by flow cytometry is initially established.

Key words: Gossypium hirsutum Linn., flow cytometry, nuclear DNA content, chromosome ploidy

WANG Ya-li, WANG Na, CHENG Hong-mei. Comparison of Methods for Rapid Determination of Cotton Ploidy by Flow Cytometry[J]. Biotechnology Bulletin, 2022, 38(12): 144-148.

Figures/Tables 1

References 23

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