Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (2): 139-146.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0616
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CUI Ji-jie1(), CAI Wen-bo1, ZHUANG Qing-hui1, GAO Ai-ping2, HUANG Jian-feng2, CHEN Ya-hui3, SONG Zhi-zhong1,3()
Received:
2022-05-16
Online:
2023-02-26
Published:
2023-03-07
CUI Ji-jie, CAI Wen-bo, ZHUANG Qing-hui, GAO Ai-ping, HUANG Jian-feng, CHEN Ya-hui, SONG Zhi-zhong. Biological Function of Gene MiISU1 for Fe-S Cluster Assembly in Mangifera indica[J]. Biotechnology Bulletin, 2023, 39(2): 139-146.
Fig. 1 Amino acid sequence identity analysis of ISU1 homologs from 13 plant species At: Arabidopsis thaliana; Bd: Brachypodium distachyon; Cs: Citrus sinensis; Es: Thellungiella salsuginea; Fv: Fragaria vesca; Gm: Glycine max; Mi: Mangifera indica; Md: Malus domestica; Os: Oryza sativa; Pp: Prunus persica; Pt: Populus trichocarpa; Sl: Solanum lycopersicum; Vi: Vitis vinifera. The same below
Fig. 4 Differential responses of MiISU1 gene to different Fe supplies in mango tissue culture seedlings * indicates significant difference at the P < 0.05 level, ** indicates extremely significant difference at the P < 0.01 level
Fig. 5 Generation and verification of MiISU1 over-expres-sion transgenic Arabidopsis seedlings A: Schematic diagram of recombinant expression vector construction; B: PCR verification of T1 transgenic lines
处理Treatment | 对照Control | 缺铁Fe depletion | ||||
---|---|---|---|---|---|---|
野生型Wild type | T3转基因株系 T3 transgenic lines | 野生型 Wild type | T3转基因株系 T3 transgenic lines | |||
整株生物鲜重Fresh weight of whole seedling/g | 60.62 ± 5.01 | 78.24 ± 5.29** | 21.75 ± 2.53 | 28.39 ± 3.21** | ||
地上部生物鲜重Fresh weight of shoots/g | 49.42 ± 3.16 | 62.52 ± 3.44** | 16.08 ± 1.92 | 15.73 ± 1.68 | ||
根部生物鲜重Fresh weight of roots/g | 10.58 ± 1.44 | 15.72 ± 1.51** | 5.67 ± 0.65 | 12.66 ± 1.47** | ||
整株铁含量 Fe concentration/(g·Kg-1 DW) | 0.32 ± 0.02 | 0.47 ± 0.04** | 0.08 ± 0.01 | 0.13 ± 0.01** | ||
叶片叶绿素含量Total leaf chlorophyll/(g·kg-1 FW) | 0.46 ± 0.03 | 0.51 ± 0.05 | 0.17 ± 0.02 | 0.23 ± 0.02** | ||
NiR活性NiR activity/(U·mg-1·protein-1) | 1.23 ± 0.11 | 1.51 ± 0.12* | 0.32 ± 0.02 | 0.34 ± 0.02 | ||
ACO活性 ACO activity/(U·mg-1·protein-1) | 0.43 ± 0.02 | 0.53 ± 0.03* | 0.17 ± 0.01 | 0.23 ± 0.01** | ||
SDH活性SDH activity/(U·mg-1·protein-1) | 6.97 ± 0.41 | 9.31 ± 0.46** | 2.17 ± 0.22 | 2.83 ± 0.25** |
Table 1 Physiological analysis of transgenic Arabidopsis seedlings under Fe depletion for 5 d
处理Treatment | 对照Control | 缺铁Fe depletion | ||||
---|---|---|---|---|---|---|
野生型Wild type | T3转基因株系 T3 transgenic lines | 野生型 Wild type | T3转基因株系 T3 transgenic lines | |||
整株生物鲜重Fresh weight of whole seedling/g | 60.62 ± 5.01 | 78.24 ± 5.29** | 21.75 ± 2.53 | 28.39 ± 3.21** | ||
地上部生物鲜重Fresh weight of shoots/g | 49.42 ± 3.16 | 62.52 ± 3.44** | 16.08 ± 1.92 | 15.73 ± 1.68 | ||
根部生物鲜重Fresh weight of roots/g | 10.58 ± 1.44 | 15.72 ± 1.51** | 5.67 ± 0.65 | 12.66 ± 1.47** | ||
整株铁含量 Fe concentration/(g·Kg-1 DW) | 0.32 ± 0.02 | 0.47 ± 0.04** | 0.08 ± 0.01 | 0.13 ± 0.01** | ||
叶片叶绿素含量Total leaf chlorophyll/(g·kg-1 FW) | 0.46 ± 0.03 | 0.51 ± 0.05 | 0.17 ± 0.02 | 0.23 ± 0.02** | ||
NiR活性NiR activity/(U·mg-1·protein-1) | 1.23 ± 0.11 | 1.51 ± 0.12* | 0.32 ± 0.02 | 0.34 ± 0.02 | ||
ACO活性 ACO activity/(U·mg-1·protein-1) | 0.43 ± 0.02 | 0.53 ± 0.03* | 0.17 ± 0.01 | 0.23 ± 0.01** | ||
SDH活性SDH activity/(U·mg-1·protein-1) | 6.97 ± 0.41 | 9.31 ± 0.46** | 2.17 ± 0.22 | 2.83 ± 0.25** |
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