Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (4): 157-165.doi: 10.13560/j.cnki.biotech.bull.1985.2022-0832

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Advances in Upstream Open Reading Frame in Plant Genes

XUE Jiao ZHU Qing-feng FENG Yan-zhao CHEN Pei LIU Wen-hua ZHANG Ai-xia LIU Qin-jian ZHANG Qi YU Yang()   

  1. Guangdong Key Laboratory for Crop Germplasm Resources Preservation and Utilization, Key Laboratory of Modern Biological Seed Industry in South China, Ministry of Agriculture and Rural Affairs, Agro-biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou 510640
  • Received:2022-07-05 Online:2023-04-26 Published:2023-05-16

Abstract:

uORF(upstream open reading frame)is a kind of mRNA element that can accurately control protein translation. It is located in the 5' leader region of mRNA. It has been reported to regulate the translation rate of downstream mORF(main open reading frame)by inhibiting the initiation of translation. Currently, the prediction and identification of plant uORF mainly focus on bioinformatics prediction and translatomics identification technology. uORF has regulatory roles on plants in many aspects, such as growth and development, nutrition and metabolism, disease resistance and immunity. In this review, we summarize the classification, functional mechanism, prediction and identification methods of plant uORF, strategies for plants to evade uORF, and the application progress of plant uORF in engineering. The purpose of this paper is to gain a more systematic and in-depth understanding of the function and mechanism of plant uORFs, and to provide a reference for the application of uORF in crop molecular breeding.

Key words: uORF, translational regulation, ribosome, growth and development, crop breeding