Advances in Plant Vacuolar Processing Enzymes

doi:10.13560/j.cnki.biotech.bull.1985.2020-1131

Abstract

Abstract:

Plant vacuolar processing enzyme(VPE)is an aspartic acid specific cysteine protease. VPE belongs to the peptidase C13 family and has substrate specificity for the carboxyl peptide chain of aspartic acid and asparagine residues. VPE is similar to caspase-1 protein from animal in both structure and function. VPE is a protease responsible for maturation and activation of vacuolar proteins in plants. It plays a multifunctional role in different plant organs and different stages of plant development and death,and mediates programmed cell death(PCD)by triggering vacuole rupture and initiating the proteolytic cascade. VPE also has peptide linking activity,which is responsible for the production of cyclic peptides in certain plants. There are four types of VPE in angiosperms during the evolution process:alpha-type VPE,beta-type VPE,gamma-type VPE,and delta-type VPE. This article summarizes the evolution,classification,maturation,activation processes and the functions of VPE in plant,as well as the latest progress and future research areas in this field,aiming to more comprehensively understand the role of VPE in plants.

Key words: vacuolar processing enzyme, programmed cell death, cysteine protease, growth and development

SU Yu, LI Zong-yun, HAN Yong-hua. Advances in Plant Vacuolar Processing Enzymes[J]. Biotechnology Bulletin, 2021, 37(6): 181-191.

Figures/Tables 4

References 92

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物种 Species	组织 Organization	VPE在不同类型PCD中的研究 Study on VPE in different types of PCD	参考文献 References
烟草	叶	液泡膜裂解介导的PCD,沉默VPE及caspase-1抑制剂能够抑制PCD及病毒增殖	[9,34-35]
	叶	VPE沉默诱导气孔关闭,抑制PCD	[36]
	花瓣	VPE基因表达的上调	[37]
	悬浮培养细胞	液泡膜裂解介导的PCD,VPE缺失及caspase-1抑制剂对PCD的抑制,VPE基因上调与VPE活性增加	[38-39]
	根	Ced-9抑制铝诱导的PCD促进植物对铝的耐受	[40]
拟南芥	花药	γ VPE基因表达的上调	[41]
	叶	液泡膜裂解介导的PCD,VPE缺失突变体及caspase-1抑制剂对PCD的抑制作用,重组p35蛋白对VPE活性的抑制作用	[42-43]
	叶	VPE缺失突变体的卵子产孢量减少,卵子感染期间γ VPE活性的增加	[44]
	叶、侧根、悬浮培养细胞	α VPE和γ VPE基因的上调	[45-46]
	叶原生质体	caspase-1抑制剂和p35超表达对PCD的抑制作用	[47]
	种皮	δ VPE突变体内珠被两层细胞PCD延迟	[48]
水稻	胚珠	VPE基因随类caspase活性的增加上调	[49]
水稻	种子	caspase-1抑制剂对PCD的抑制使VPE活性提高	[44]
土豆	块茎顶芽分生组织	caspase-1抑制剂抑制PCD使VPE活性提高	[50]
苹果	叶	VPE基因的表达在HR过程中上调	[51]
番茄	叶	Bcl-2过表达下调VPE基因表达,在抑制NaCl诱导的PCD过程中起重要作用	[52-53]
番茄	悬浮培养细胞	caspase-1抑制剂对PCD的抑制作用	[54]
大豆	叶原生质体	VPE基因上调,鉴定两种VPE基因表达转录因子	[55]
甘薯	叶、根	参与发育、衰老、生殖过程中的PCD	[56]

物种 Species	组织 Organization	VPE在不同类型PCD中的研究 Study on VPE in different types of PCD	参考文献 References
烟草	叶	液泡膜裂解介导的PCD,沉默VPE及caspase-1抑制剂能够抑制PCD及病毒增殖	[9,34-35]
	叶	VPE沉默诱导气孔关闭,抑制PCD	[36]
	花瓣	VPE基因表达的上调	[37]
	悬浮培养细胞	液泡膜裂解介导的PCD,VPE缺失及caspase-1抑制剂对PCD的抑制,VPE基因上调与VPE活性增加	[38-39]
	根	Ced-9抑制铝诱导的PCD促进植物对铝的耐受	[40]
拟南芥	花药	γ VPE基因表达的上调	[41]
	叶	液泡膜裂解介导的PCD,VPE缺失突变体及caspase-1抑制剂对PCD的抑制作用,重组p35蛋白对VPE活性的抑制作用	[42-43]
	叶	VPE缺失突变体的卵子产孢量减少,卵子感染期间γ VPE活性的增加	[44]
	叶、侧根、悬浮培养细胞	α VPE和γ VPE基因的上调	[45-46]
	叶原生质体	caspase-1抑制剂和p35超表达对PCD的抑制作用	[47]
	种皮	δ VPE突变体内珠被两层细胞PCD延迟	[48]
水稻	胚珠	VPE基因随类caspase活性的增加上调	[49]
水稻	种子	caspase-1抑制剂对PCD的抑制使VPE活性提高	[44]
土豆	块茎顶芽分生组织	caspase-1抑制剂抑制PCD使VPE活性提高	[50]
苹果	叶	VPE基因的表达在HR过程中上调	[51]
番茄	叶	Bcl-2过表达下调VPE基因表达,在抑制NaCl诱导的PCD过程中起重要作用	[52-53]
番茄	悬浮培养细胞	caspase-1抑制剂对PCD的抑制作用	[54]
大豆	叶原生质体	VPE基因上调,鉴定两种VPE基因表达转录因子	[55]
甘薯	叶、根	参与发育、衰老、生殖过程中的PCD	[56]