Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (5): 217-223.doi: 10.13560/j.cnki.biotech.bull.1985.2022-1231

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Expression and Function Analysis of Transcription Factor PcMYB2 from Polygonum cuspidatum

JIANG Qing-chun(), DU Jie, WANG Jia-cheng, YU Zhi-he, WANG Yun, LIU Zhong-yu()   

  1. College of Life Science, Yangtze University, Jingzhou 434025
  • Received:2022-10-08 Online:2023-05-26 Published:2023-06-08
  • Contact: LIU Zhong-yu E-mail:2870140725@qq.com;zyliu2004@126.com

Abstract:

This work aims to identify the transcriptional activity and expression profile of PcMYB2, encoding an R2R3-MYB transcription factor from Polygonum cuspidatum, and to evaluate the biological functions of PcMYB2 in transgenic Arabidopsis thaliana plant and transformed P. cuspidatum hairy roots. The yeast one hybrid was used to analyze transcriptional activity. And quantitative real time PCR(RT-qPCR)technique was applied to detect the tissue-specific expression profile of PcMYB2 in P. cuspidatum and its expression pattern in responses to stress treatments. The DMACA method was to determine the contents of proanthocyanidins in PcMYB2-transgenic A. thaliana and P. cuspidatum hairy roots. The yeast one hybrid experiment showed that PcMYB2 had transcriptional activity. The PcMYB2 gene was expressed in the roots, stems and leaves of P. cuspidatum, with the highest expression in the leaves, and the expression of PcMYB2 gene in the leaves was induced after application of ABA or H2O2. Transgenic A. thaliana presented an increase in the pigmentation of mature seed compared with the wild type, and the proanthocyanidins content was 1.8-fold higher than that of the wild type. Staining of the hairy roots with DMACA was darker compared with wild type, and proanthocyanidins content was 2.3-fold higher. Altogether, this study reveals that PcMYB2 has transcriptional activity and may promote the proanthocyanidins biosynthesis.

Key words: Polygonum cuspidatum Sieb. et Zucc., PcMYB2, transcriptional activity, expression analysis, proanthocyanidins biosynthesis