Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (11): 297-307.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0324
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MAO Ke-xin1,2(), WANG Hai-rong1, AN Miao1, LIU Teng-fei1, WANG Shi-jin3, LI Jian1(), LI Guo-tian1()
Received:
2023-04-07
Online:
2023-11-26
Published:
2023-12-20
Contact:
LI Jian, LI Guo-tian
E-mail:mao_kx@foxmail.com;lijian097597@163.com;ligt2008@163.com
MAO Ke-xin, WANG Hai-rong, AN Miao, LIU Teng-fei, WANG Shi-jin, LI Jian, LI Guo-tian. Identification of GRAS Gene Family and Expression Analysis Under Low Temperature Stress in Actinidia chinensis[J]. Biotechnology Bulletin, 2023, 39(11): 297-307.
基因Gene | 正向引物Forward primer(5'-3') | 反向引物Reverse primer(5'-3') |
---|---|---|
AcGRAS32 | ACATTAGGCTCCCACCACTG | CTCCCGAGTCATTCAACCAT |
AcGRAS3 | AGTTCCGGATGTTGAGGTTG | TTTGAAGCGGGGTAAGAATG |
AcGRAS53 | TTCCAATCCTGCAATGAACA | AGGAATGATGCGGATCTGAC |
AcGRAS79 | GGTCGATGATCTTTCCGTGT | TCCCGAAGAGCTTCAGAAAA |
AcGRAS6 | GAACGAAGGTTTTCGCTCTG | ACTGGATCCCCTGAGCTTTT |
AcGRAS42 | AGTCGGCGATGAGCTTAAAA | GGAACCTTGTACAGCCGAAA |
Actin | GTGCTCAGTGGTGGTTCAA | GACGCTGTATTTCCTCTCAG |
Table 1 Primers used for quantitative real-time PCR
基因Gene | 正向引物Forward primer(5'-3') | 反向引物Reverse primer(5'-3') |
---|---|---|
AcGRAS32 | ACATTAGGCTCCCACCACTG | CTCCCGAGTCATTCAACCAT |
AcGRAS3 | AGTTCCGGATGTTGAGGTTG | TTTGAAGCGGGGTAAGAATG |
AcGRAS53 | TTCCAATCCTGCAATGAACA | AGGAATGATGCGGATCTGAC |
AcGRAS79 | GGTCGATGATCTTTCCGTGT | TCCCGAAGAGCTTCAGAAAA |
AcGRAS6 | GAACGAAGGTTTTCGCTCTG | ACTGGATCCCCTGAGCTTTT |
AcGRAS42 | AGTCGGCGATGAGCTTAAAA | GGAACCTTGTACAGCCGAAA |
Actin | GTGCTCAGTGGTGGTTCAA | GACGCTGTATTTCCTCTCAG |
Fig. 10 Analysis of GRAS gene expression in kiwifruit during overwintering 1-3 represents kiwifruit branches in overwintering period on November 15, 2020, January 15, 2021 and March 15, 2021 respectively
Fig. 11 Expression analysis of 6 kiwifruit GRAS genes under low temperature treatment Different letters indicate that there are significant differences in genes at the level of 0.05 under different treatment times. *, ** indicated significant difference between treatment and control at 0.05 and 0.01 levels
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