Biotechnology Bulletin ›› 2023, Vol. 39 ›› Issue (11): 297-307.doi: 10.13560/j.cnki.biotech.bull.1985.2023-0324

Previous Articles     Next Articles

Identification of GRAS Gene Family and Expression Analysis Under Low Temperature Stress in Actinidia chinensis

MAO Ke-xin1,2(), WANG Hai-rong1, AN Miao1, LIU Teng-fei1, WANG Shi-jin3, LI Jian1(), LI Guo-tian1()   

  1. 1. Shandong Institute of Pomology, Tai'an 271000
    2. School of Life Science, Yunnan University, Kunming 650000
    3. Northern Kiwi Fruit(Rizhao)Technology Research Institute, Rizhao 276500
  • Received:2023-04-07 Online:2023-11-26 Published:2023-12-20
  • Contact: LI Jian, LI Guo-tian E-mail:mao_kx@foxmail.com;lijian097597@163.com;ligt2008@163.com

Abstract:

GRAS gene family is widely involved in plant growth and stress response. Low temperature is one of the important factors that restricts the production and distribution of kiwifruit(Actinidia chinensis). The GRAS gene family of kiwifruit was identified, its expression under low temperature stress was analyzed, which may provide a theoretical basis for the research on cold resistance and variety breeding of kiwifruit. The GRAS gene family conserved domains were compared with the genome of A. chinensis ‘Hongyang’ reference, and the identified family members were analyzed by phylogenetic tree, protein physical, chemical properties, gene structure, protein tertiary structure, protein motif, cis-acting elements, collinearity, codon usage preference and gene expression mode. The results showed that there was a total of 79 GRAS family genes in kiwifruit genome, belonging to 8 subfamilies, and there were differences in gene and protein structures among each subfamily. Analysis of cis-acting elements showed the genes were involved in multiple plant hormone, growth and development, and stress response. Through codon preference analysis, it was found that the third base of GRAS family preferred to use pyrimidine bases G/T. Six genes were identified that may be involved in the low-temperature stress process in kiwifruit, and this hypothesis was validated by fluorescence quantitative PCR.

Key words: kiwifruit, GRAS gene family, codon analysis, low temperature stress