Identification and Tissue Expression of Four Transcription Factors in Pholidota chinensis

doi:10.13560/j.cnki.biotech.bull.1985.2023-1096

Abstract

Abstract:

【Objective】Pholidota chinensis Lindl is a rare and endangered epiphytic orchid. The epiphytes have evolved many special morphological structures and physiological and biochemical characteristics compared to geophytes. The transcription factors play a role in regulating plant growth and development. The aim of this paper is to explore and discover transcription factors in P. chinensis that regulate its growth and development.【Method】This study used PacBio and Illumina sequencing techniques to obtain the full-length transcriptome data of P. chinensis and the gene expressions in the roots, rhizomes, pseudobulbs and leaves. After further bioinformatics analysis, the four transcription factor family members, physicochemical properties and tissue expression specificity of P. chinensis were revealed. The expression of C2H2 family and bHLH family was analyzed by RT-qPCR using sodium chloride（NaCl）and methyl jasmonate（MeJA）induced adversity stresses.【Result】The four identified transcription factors and their families members in P. chinensis were: 21 members in C2H2 family, 27 members in bHLH family, 30 members in WRKY family, and 19 members in bZIP family. These four families and their members were mainly hydrophilic unstable proteins, and their subcellular localization was mainly located in the nucleus. Some members of bHLH families were located in cytoplasm, chloroplast and other organelles. The gene family had typical conserved domain characteristics, and showed obvious tissue specificity in the roots, rhizomes, pseudobulbs and leaves of P. chinensis. Expressions of all genes in C2H2 families and bHLH families were affected by NaCl and MeJA, but family members differed in whether they were promoted or repressed in the expression and in the amount of their expression.【Conclusion】The number of four transcription factor family members in P. chinensis was identified, and the members of each family are mainly hydrophilic unstable proteins located in the nucleus with conserved structural domain features and tissue expression specificity.

Key words: Pholidota chinensis, C2H2, bHLH, WRKY, bZIP, physical and chemical properties, tissue expression

LIU Bao-cai, ZHANG Wu-jun, ZHAO Yun-qing, HUANG Ying-zhen, CHEN Jing-ying. Identification and Tissue Expression of Four Transcription Factors in Pholidota chinensis[J]. Biotechnology Bulletin, 2024, 40(5): 141-152.

Figures/Tables 6

Fig. 1 Number of top 30 transcription factor families

Fig. 2 Characteristics of C2H2 gene family in P. chinensis A: Conserved motifs in C2H2 family; B: differential expressions of C2H2 family in different tissues; C: C2H2 family evolution

Fig. 3 Characteristics of bHLH gene family in P. chinensis A: Conserved motifs in bHLH family. B: Differential expressions of bHLH family in different tissues. C: bHLH family evolution

Fig. 4 Characteristics of WRKY gene family in P. chinensis A: Conserved motifs of WRKY family. B: WRKY family evolution. C: Differential expressions of WRKY family in different tissues

Fig. 5 Characteristics of bZIP gene family in P. chinensis A: Conserved motifs of bZIP family; B: bZIP family evolution; C: differential expressions of bZIP family in different tissues

Fig. 6 Expression analysis of C2H2 (A) and bHLH (B) families in P. chinensis under NaCl and MeJA stresses

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