Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (2): 245-252.doi: 10.13560/j.cnki.biotech.bull.1985.2022-1508
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LI Can(), JIANG Xiang-ning, GAI Ying()
Received:
2022-12-09
Online:
2024-02-26
Published:
2024-03-13
Contact:
GAI Ying
E-mail:2516134421@qq.com;gaiying@bjfu.edu.cn
LI Can, JIANG Xiang-ning, GAI Ying. Cloning of the LkF3H2 Gene in Larix kaempferi and Its Function in Regulating Flavonoid Metabolism[J]. Biotechnology Bulletin, 2024, 40(2): 245-252.
Fig. 3 LkF3H2 protein secondary and tertiary structure prediction, conserved domain prediction A: Secondary structure prediction. B and C: Tertiary structure prediction. D: Conserved domain prediction
Fig. 5 Expressions of LkF3H2 gene in different tissues in Larix kaempferi Lk-1: One-month seedling. Lk-2: One-year seedling. The data are analyzed with Tukey’s multiple comparisons test and shown as the mean ± SE of three independent replicates. * and ** indicate significant differences at 0.05 and 0.01 levels
Fig. 6 Growth and development stages of transgenic tobacco A: Pre-culture. B: Regeneration culture. C: Rooting culture. D: Transgenic tobacco. E: PCR identification result
Fig. 7 Expression of the LkF3H2 gene and flavonoid content in transgenic tobacco A: Expression of the LkF3H2 gene. B: Flavonoid content of transgenic tobacco. C: Relationship between the expression of the LkF3H2 gene and flavonoid content. The data are analyzed with Tukey’s multiple comparisons test and shown as the mean ± SE of three independent replicates. * and ** indicate significant differences at 0.05 and 0.01 levels
[1] |
Sum TJ, Sum TH, Galloway WRJD, et al. Synthesis of structurally diverse biflavonoids[J]. Tetrahedron, 2018, 74(38): 5089-5101.
doi: 10.1016/j.tet.2018.05.003 URL |
[2] |
Perez-Vizcaino F, Fraga CG. Research trends in flavonoids and health[J]. Arch Biochem Biophys, 2018, 646: 107-112.
doi: S0003-9861(18)30147-4 pmid: 29580946 |
[3] |
Dai MJ, Kang XR, Wang YQ, et al. Functional characterization of flavanone 3-hydroxylase(F3H)and its role in anthocyanin and flavonoid biosynthesis in mulberry[J]. Molecules, 2022, 27(10): 3341.
doi: 10.3390/molecules27103341 URL |
[4] |
Jiang F, Wang JY, Jia HF, et al. RNAi-mediated silencing of the flavanone 3-hydroxylase gene and its effect on flavonoid biosynthesis in strawberry fruit[J]. J Plant Growth Regul, 2013, 32(1): 182-190.
doi: 10.1007/s00344-012-9289-1 URL |
[5] |
Kim EG, Yun S, Park JR, et al. Identification of F3H, major secondary metabolite-related gene that confers resistance against whitebacked planthopper through QTL mapping in rice[J]. Plants, 2021, 10(1): 81.
doi: 10.3390/plants10010081 URL |
[6] |
Aguadé M. Nucleotide sequence variation at two genes of the phenylpropanoid pathway, the FAH1 and F3H genes, in Arabidopsis thaliana[J]. Mol Biol Evol, 2001, 18(1): 1-9.
pmid: 11141187 |
[7] |
Jiang F, Chen XP, Hu WS, et al. Identification of differentially expressed genes implicated in peel color(red and green)of Dimocarpus confinis[J]. SpringerPlus, 2016, 5(1): 1088.
doi: 10.1186/s40064-016-2743-y pmid: 27468388 |
[8] |
He Y, Luo Y, Wang Q, et al. Spray treatment of leaves with Fe2+ promotes procyanidin biosynthesis by upregulating the expression of the F3H and ANS genes in red rice grains(Oryza sativa L.)[J]. J Cereal Sci, 2021, 100: 103231.
doi: 10.1016/j.jcs.2021.103231 URL |
[9] |
孙蓉, 刘姗, 高静雷. 三角梅黄烷酮3-羟化酶基因的克隆及表达分析[J]. 生物技术通报, 2022, 38(11): 122-128.
doi: 10.13560/j.cnki.biotech.bull.1985.2022-0048 |
Sun R, Liu S, Gao JL. Cloning and expression analysis of flavanone 3-hydroxylase gene from Bougainvillea spectabilis[J]. Biotechnol Bull, 2022, 38(11): 122-128. | |
[10] |
Han YH, Huang KY, Liu YJ, et al. Functional analysis of two flavanone-3-hydroxylase genes from Camellia sinensis: a critical role in flavonoid accumulation[J]. Genes, 2017, 8(11): 300.
doi: 10.3390/genes8110300 URL |
[11] | 郑晟, 毛玉珊, 张腾国, 等. 柠条锦鸡儿F3H基因克隆及功能分析[J]. 广西植物, 2017, 37(6): 723-733. |
Zheng S, Mao YS, Zhang TG, et al. Cloning and expression analysis of F3H gene in Caragana korshinskii[J]. Guihaia, 2017, 37(6): 723-733. | |
[12] | 朱顺华, 钟秀来, 王堃, 等. 野生胡萝卜和栽培胡萝卜中DcF3H 基因的克隆、进化及功能分析[J/OL]. 分子植物育种, 2022. http://kns.cnki.net/kcms/detail/46.1068.s.20220719.1055.002.html. |
Zhu SH, Zhong XL, Wang K, et al. Cloning and phylogenetic and functional analysis of DcF3H Gene in wild carrots and cultivated carrots[J/OL]. Molecular Plant Breeding, 2022. http://kns.cnki.net/kcms/detail/46.1068.s.20220719.1055.002.html. | |
[13] |
张慧荣, 赵萍萍, 冯杰, 等. 长叶红砂黄烷酮-3-羟基转移酶3基因(RtF3H3)的克隆、表达分析及酵母表达载体的构建[J]. 华北农学报, 2015, 30(5): 45-50.
doi: 10.7668/hbnxb.2015.05.008 |
Zhang HR, Zhao PP, Feng J, et al. Cloning, expressing patterns and constructing yeast expression vectors of flavanone-3-hydroxylase3(RtF3H3)from Reaumuria trigyna[J]. Acta Agric Boreali Sin, 2015, 30(5): 45-50. | |
[14] | 陈可欣, 乔中全, 曾慧杰, 等. 紫薇F3H基因的克隆与生物信息学分析[J]. 湖南林业科技, 2021, 48(2): 55-60. |
Chen KX, Qiao ZQ, Zeng HJ, et al. Cloning and bioinformatics analysis of F3H gene in Lagerstroemia indica[J]. Hunan For Sci Technol, 2021, 48(2): 55-60. | |
[15] |
Inoue M, Horimoto K. Relationship between regulatory pattern of gene expression level and gene function[J]. PLoS One, 2017, 12(5): e0177430.
doi: 10.1371/journal.pone.0177430 URL |
[16] |
Chawla K, Sinha K, Neelam, et al. Identification and functional characterization of two acyl CoA: diacylglycerol acyltransferase 1(DGAT1)genes from forage Sorghum(Sorghum bicolor)embryo[J]. Phytochemistry, 2020, 176: 112405.
doi: 10.1016/j.phytochem.2020.112405 pmid: 32473393 |
[17] |
Khumkarjorn N, Thanonkeo S, Yamada M, et al. Cloning and expression analysis of a flavanone 3-hydroxylase gene in Ascocenda orchid[J]. J Plant Biochem Biotechnol, 2017, 26(2): 179-190.
doi: 10.1007/s13562-016-0379-1 URL |
[18] |
Shoeva OY, Khlestkina EK. F3h gene expression in various organs of wheat[J]. Mol Biol, 2013, 47(6): 901-903.
doi: 10.1134/S0026893313060137 URL |
[19] |
Ren YJ, Mao HY, Su WH, et al. Improved drought and salt tolerance in transgenic Nicotiana benthamiana by overexpressing sugarcane ScSEC14p gene[J]. Sugar Tech, 2021, 23(2): 326-335.
doi: 10.1007/s12355-020-00895-y |
[20] |
Si C, Dong W, Teixeira da Silva JA, et al. Functional analysis of flavanone 3-hydroxylase(F3H)from Dendrobium officinale, which confers abiotic stress tolerance[J]. Hortic Plant J, 2023, 9(2): 356-364.
doi: 10.1016/j.hpj.2022.03.006 URL |
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