Biotechnology Bulletin ›› 2024, Vol. 40 ›› Issue (7): 78-89.doi: 10.13560/j.cnki.biotech.bull.1985.2024-0020
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ZHANG Zhen-yu1,2,3(), JIN Li-wu1,2,3, WANG Jing-zun1,2,3, TIAN Ling1,2,3, QIAO Zi-lin1,2,4, YANG Di1,2,5, AYIMUGULI Abudureyimu1,3()
Received:
2024-01-05
Online:
2024-07-26
Published:
2024-07-30
Contact:
AYIMUGULI Abudureyimu
E-mail:375470311@qq.com;Ayimgul80@xbmu.edu.cn
ZHANG Zhen-yu, JIN Li-wu, WANG Jing-zun, TIAN Ling, QIAO Zi-lin, YANG Di, AYIMUGULI Abudureyimu. Research Progress in the Immortalization of Animal Cells[J]. Biotechnology Bulletin, 2024, 40(7): 78-89.
物种 Species | 细胞系名称 Cell line name | 细胞系来源 Cell line source | 参考文献 Reference |
---|---|---|---|
兔 Rabbit | HIG-82 | 兔膝关节成纤维细胞 Rabbit knee joint fibroblasts | [ |
人 Human | ECV304 | 人脐静脉内皮细胞Human umbilical vein endothelial cells | [ |
兔 Rabbit | RCE1 | 兔角膜上皮细胞 Rabbit corneal epithelial cells | [ |
兔 Rabbit | iRCEC | 兔角膜内皮细胞 Rabbit corneal endothelial cells | [ |
鸡 Chicken | DF-1 | 鸡胚胎成纤维细胞 Chicken embryo fibroblasts | [ |
豚鼠 Guinea pig | GPK- si | 豚鼠肾细胞 Guinea pig kidney cells | [ |
Table 1 Translation of spontaneously immortalized cell lines
物种 Species | 细胞系名称 Cell line name | 细胞系来源 Cell line source | 参考文献 Reference |
---|---|---|---|
兔 Rabbit | HIG-82 | 兔膝关节成纤维细胞 Rabbit knee joint fibroblasts | [ |
人 Human | ECV304 | 人脐静脉内皮细胞Human umbilical vein endothelial cells | [ |
兔 Rabbit | RCE1 | 兔角膜上皮细胞 Rabbit corneal epithelial cells | [ |
兔 Rabbit | iRCEC | 兔角膜内皮细胞 Rabbit corneal endothelial cells | [ |
鸡 Chicken | DF-1 | 鸡胚胎成纤维细胞 Chicken embryo fibroblasts | [ |
豚鼠 Guinea pig | GPK- si | 豚鼠肾细胞 Guinea pig kidney cells | [ |
细胞 Cells | 传代次数 Cell passages | 细胞系名称 Cell line name | 永生化方式 Immortalization method | 生物学特性 Biological characteristics |
---|---|---|---|---|
猪精原干细胞 Porcine spermatogonial stem cells | >35代 Over 35 passages | Ttag and Puro | SV40LT | 未出现形态改变及多核细胞[ |
人角膜干细胞 Human corneal stromal stem cells | >15代 Over 15 passages | SV40T-imCSSC | SV40LT | 保留干细胞特性,具有抗炎特性[ |
小鼠肠平滑肌细胞 Mouse intestinal smooth muscle cells | 至50代 Up to 50 passages | ISMC- Hc | SV40LT | 与原代肠平滑肌细胞(ISMC)特征基本一致,增殖活性更强,且无致瘤性[ |
人牙髓干细胞 Human dental pulp stem cells | >15代 Over 15 passages | TERT | hTERT | 增殖速度增快,且维持干细胞特性[ |
胎牛皮肤成纤维细胞 Fetal bovine skin fibroblasts | >50代 Over 50 passages | hTERT-CSF | hTERT | 增殖速度更快,且无多核细胞[ |
猪耳、胎儿和肺组织源成纤维细胞 Primary fibroblasts prepared from pig ear, fetal and lung tissues | 30-45代 30-45 passages | Fibroblast cell line | hTERT | 无细胞特性改变,增值代次增加,但SLA I类基因略有上调[ |
猪肺源巨噬细胞 Porcine lung-derived monocyte-derived macrophages | >54代 Over 54 passages | IPLuM | SV40LT和pTERT | 保持巨噬细胞形态,对ASFV易感[ |
猪小肠巨噬细胞 Porcine intestinal macrophages | >50代 Over 50 passages | IPIM | SV40LT和pTERT | 保留巨噬细胞特征,对PRRSV敏感性增加[ |
牦牛瘤胃上皮细胞 Epithelial cells of the rumen stomach of yaks | >30代 Over 30 passages | SV40T-YREC-hTERT | SV40LT和hTERT | 保留上皮细胞特性,无多核细胞[ |
Table 2 SV40, TERT-induced immortalized cell lines
细胞 Cells | 传代次数 Cell passages | 细胞系名称 Cell line name | 永生化方式 Immortalization method | 生物学特性 Biological characteristics |
---|---|---|---|---|
猪精原干细胞 Porcine spermatogonial stem cells | >35代 Over 35 passages | Ttag and Puro | SV40LT | 未出现形态改变及多核细胞[ |
人角膜干细胞 Human corneal stromal stem cells | >15代 Over 15 passages | SV40T-imCSSC | SV40LT | 保留干细胞特性,具有抗炎特性[ |
小鼠肠平滑肌细胞 Mouse intestinal smooth muscle cells | 至50代 Up to 50 passages | ISMC- Hc | SV40LT | 与原代肠平滑肌细胞(ISMC)特征基本一致,增殖活性更强,且无致瘤性[ |
人牙髓干细胞 Human dental pulp stem cells | >15代 Over 15 passages | TERT | hTERT | 增殖速度增快,且维持干细胞特性[ |
胎牛皮肤成纤维细胞 Fetal bovine skin fibroblasts | >50代 Over 50 passages | hTERT-CSF | hTERT | 增殖速度更快,且无多核细胞[ |
猪耳、胎儿和肺组织源成纤维细胞 Primary fibroblasts prepared from pig ear, fetal and lung tissues | 30-45代 30-45 passages | Fibroblast cell line | hTERT | 无细胞特性改变,增值代次增加,但SLA I类基因略有上调[ |
猪肺源巨噬细胞 Porcine lung-derived monocyte-derived macrophages | >54代 Over 54 passages | IPLuM | SV40LT和pTERT | 保持巨噬细胞形态,对ASFV易感[ |
猪小肠巨噬细胞 Porcine intestinal macrophages | >50代 Over 50 passages | IPIM | SV40LT和pTERT | 保留巨噬细胞特征,对PRRSV敏感性增加[ |
牦牛瘤胃上皮细胞 Epithelial cells of the rumen stomach of yaks | >30代 Over 30 passages | SV40T-YREC-hTERT | SV40LT和hTERT | 保留上皮细胞特性,无多核细胞[ |
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