Review of Marker-free Transgenic Crop

Abstract

Abstract: Selectable marker genes（SMGs）based on antibiotic or herbicide resistance have been widely used to identify ‘true’transformants. To release the public concerns for the bio-safety of SMGs，a new strategy has been developed to obtain marker-free transgenicplants to eliminate the marker gene from the nuclear or chloroplast genome after selection. Marker-free transgenic crops have a lot of uniqueadvantage such as reducing the trepidations from the SMGs and transferring exogenous genes gradually into transgenic crops. In this review，different approaches of marker-free transgenic method were discussed.

Key words: Selectable marker genes（SMGs）, Site-specific recombination, Transposons

Chen Yangxun, Zhang Zhiguo, Lu Tiegang. Review of Marker-free Transgenic Crop[J]. Biotechnology Bulletin, 2012, 0(12): 1-7.

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