Cloning and Expression Analysis of CeActin Homologous Gene from Cymbidium ensifolium

Abstract

Abstract: The full-length cDNA sequence of Actin gene from the bud of Cymbidium ensifolium was cloned（GenBank accessionnumber: JN613147）by using RT-PCR and RACE with degenerate primer which were synthesized based on the high homologous region amongthe sequences of several monocotyledon Actin genes. The full length cDNA of CeActin was 1 434 bp with an ORF of 1 134 bp encoding a proteinwith 377 amino acids. Homology search showed that this gene shared high identity with other Actins. Semi-quantitative RT-PCR analysis showedthat the expression of CeActin had no notable difference in different tissues and different floral development stages. The results laid basis foridentification of gene transcription level in Cymbidium ensifolium using CeActin as internal control.

Key words: Cymbidium ensifolium, Actin gene, Cloning, Expression analysis

Wu Jinghua, Wu Shaohua, Yang Chao . Cloning and Expression Analysis of CeActin Homologous Gene from Cymbidium ensifolium[J]. Biotechnology Bulletin, 2012, 0(12): 88-92.

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