Abstract: A rapid and high efficient screening method of GM crop seeds is the important technical support for strengthening regulationof the agricultural GMO. In this paper, a rapid screening method of GM maize seeds was established by integrating the DNA extraction method ofhot alkaline and the multiplex real-time fluorescent PCR technology. The results showed that the rapid screening method can not only ensure thedetection accuracy and sensitivity, but also greatly reducing false-positive incidence and improve the efficiency of the GMO screening, and whichis suitable for the rapid detection of large numbers of samples.

Key words: Transgenic , Corn seed , Rapid , Screening method