Application of Synthetic Biology Based Whole-cell Biosensor Technology in the Rapid Detection of Food Safety

doi:10.13560/j.cnki.biotech.bull.1985.2022-0389

Abstract

Abstract:

Synthetic biology-based whole-cell sensing technology provides an alternative approach for rapid and on-site detection of heavy metals. Because the intracellular environment is relatively stable, whole-cell biosensor presents strong anti-interference ability; the biosensor cells can proliferate with cell self-replication, so the whole-cell biosensor has the characteristics of simple, cheap and fast in production. Therefore, whole-cell biosensor demonstrates a promising application prospect in the rapid detection of food pollutants. This article, the composition, construction methods, and types of core elements for whole-cell biosensor are summarized. Then synthetic biology-based gene circuits for multi-function detection technologies are introduced; further the commercialized applications of whole-cell biosensor in rapid food safety testing are enumerated; and finally the challenges and development prospects of cell biosensors in the rapid detection of food safety are illustrated.

Key words: synthetic biology, whole-cell biosensor, food safety, rapid detection

CHEN Xiao-lin, LIU Yang-er, XU Wen-tao, GUO Ming-zhang, LIU Hui-lin. Application of Synthetic Biology Based Whole-cell Biosensor Technology in the Rapid Detection of Food Safety[J]. Biotechnology Bulletin, 2023, 39(1): 137-149.

Figures/Tables 4

References 78

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类别Type	靶标Target	感应元件Sensing element	参考文献Reference
重金属 Heavy metals	汞Hg	MerR	[5]
	镉Cd	CadR	[6]
	砷As	ArsR	[7]
	铅Pb	PbrR	[8]
	铜Cu	CueR	[9]
农药残留 Pesticide residues	百草枯Paraquat	PqrR, OxyR, SoxR, FphR	[10]
	杀螟虫Fenitrothion	DmpR	[11]
	对硫磷、甲基对硫磷Parathion and Methyl parathion	DmpR	[12]
	阿特拉津Atrazine	（GST）	[13]
兽药残留 Veterinary residues	强力霉素Doxycycline	TetR	[14]
	四环素Tetracycline	TetR, tc-riboswitch	[15]
	红霉素Erythromycin	MphR	[16]
	庆大霉素Gentamicin	IcaR	[17]
	林可霉素Lincomycin	LmrA	[17]
	新霉素Novobiocin	neo-riboswitch	[18]
	土霉素Oxytetracycline	OtrR	[19]
	弗吉尼亚霉素 Virginiamycin	VarR	[20]
	杆菌肽Gramicidin	BcrR	[21]
食品添加剂 Food additives	苯甲酸、苯甲酸盐Benzoic acid andBenzoate	BenR	[22]
食品添加剂 Food additives	己二酸Betulonic acid	PcaR	[23]
生物性污染 Biological pollution	病原微生物 Pathogenic microorganisms	QscR	[24]

类别Type	靶标Target	感应元件Sensing element	参考文献Reference
重金属 Heavy metals	汞Hg	MerR	[5]
	镉Cd	CadR	[6]
	砷As	ArsR	[7]
	铅Pb	PbrR	[8]
	铜Cu	CueR	[9]
农药残留 Pesticide residues	百草枯Paraquat	PqrR, OxyR, SoxR, FphR	[10]
	杀螟虫Fenitrothion	DmpR	[11]
	对硫磷、甲基对硫磷Parathion and Methyl parathion	DmpR	[12]
	阿特拉津Atrazine	（GST）	[13]
兽药残留 Veterinary residues	强力霉素Doxycycline	TetR	[14]
	四环素Tetracycline	TetR, tc-riboswitch	[15]
	红霉素Erythromycin	MphR	[16]
	庆大霉素Gentamicin	IcaR	[17]
	林可霉素Lincomycin	LmrA	[17]
	新霉素Novobiocin	neo-riboswitch	[18]
	土霉素Oxytetracycline	OtrR	[19]
	弗吉尼亚霉素 Virginiamycin	VarR	[20]
	杆菌肽Gramicidin	BcrR	[21]
食品添加剂 Food additives	苯甲酸、苯甲酸盐Benzoic acid andBenzoate	BenR	[22]
食品添加剂 Food additives	己二酸Betulonic acid	PcaR	[23]
生物性污染 Biological pollution	病原微生物 Pathogenic microorganisms	QscR	[24]