Abstract: The research was designed to study the expression of Zearalenone(ZEN)mimicking epitope by p Ⅷ phage display systemand verify its reactogenicity. An expression vector pC89-CZEN was constructed by linking the oligonucleotide contained ZEN mimicking epitopesequence and Enterokinase cleavage sites with the pC89S4 phagemid which had been double digested by EcoR I and BamH I. Engineered bacteriapC89-ek-zen was obtained after pC89-CZEN being transformed into competent XL1-Blue cells. The phage particles displaying Zearalenonemimicking peptide were acquired after pC89-ek-zen being super infected by KM13 phage. The optimal expression conditions were also explored.The reactogenicity was detected by ELISA. ZEN antibody binding capacity(before and after Enterokinase digestion)were also compared. Theresults show that the expression vector pC89-CZEN was constructed successfully. The optimal expression conditions were explored by ELISA.The binding effect is influenced by bacteria concentration when helper phage KM13 and IPTG added, and the final concentration of IPTG in themedium, induced expression temperature and time. The binding efficiency after enzyme digestion was higher than before.

Key words: Phage display system , Zearalenone , Mimicking epitope , Enterokinase