Combined Hepatotoxicity Assessment of Mycotoxins AFB1 and Zearalenone on Hepatocellular Carcinoma Cells HepG2 in vitro and Its Mechanisms

doi:10.13560/j.cnki.biotech.bull.1985.2018-0588

Abstract

Abstract: Based on key apoptotic genes,early apoptosis signal indexes and apoptosis,this study used hepatocellular carcinoma Cells HepG2 cells to estimate combined hepatotoxic effects of aflatoxin B1（AFB1）and zearalenone（ZEN）and to explore its feasible mechanisms. The results showed that the combined effects of 1-10 μg/mL mycotoxins AFB1 and ZEN on the cells was synergetic,and the effect presented as weak antagonistic to toxic and further to strong synergetic with the increase of concentration. The early apoptosis indicator ROS rose with the exposure time prolonging;while GSH and MMP gradually was decreasing,and the change was the most significantly at 0 - 3 h. The ratio of Bax/Caspase-3 expression increased with the prolonging of the time;while the expressions of CYP1A2,CYP1A4 and CYP1A6 tended to be down,which was more obvious with the increase of the time. AFB1 and ZEN induced the combined hepatotoxicity via the interaction and regulation of gene Bax,Caspases-3,and CY450.

Key words: aflatoxin B1, zearalenone, high content screening, combined hepatotoxicity, combination index（CI）-isobologram equation

REN Ya-lin, LI Yun, WU Jing. Combined Hepatotoxicity Assessment of Mycotoxins AFB1 and Zearalenone on Hepatocellular Carcinoma Cells HepG2 in vitro and Its Mechanisms[J]. Biotechnology Bulletin, 2018, 34(11): 160-167.

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