Enhanced Carotenoids Production by a Rhodotorula sp. Under Stress Conditions

Abstract

Abstract: Carotenoids, displaying yellow, orange, and red color, are a series of polyene compounds with important physiologicalhealthy function. They are found widely in microorganisms, including algae and plants. The effects of four factors, including low temperature,treatment time at low temperature, acid treatment and high-concentration salt treatment, on the carotenoids production by a Rhodotorula sp. wereinvestigated. Based on this, response surface analysis method was used to elucidate the interaction of the four factors. The results showed thatthe effects of low temperature, acid, high-concentration salt treatment and the interactive effects of low temperature-acid, low temperature- highconcentrationsalt, treatment time at low temperature-acid on carotenoids production are significant. The maximum carotenoids yield of 31.04 mg/Lwas obtained at the low temperature of 15 ℃ for 48 h, with pH 3.5 and 2 mol/L NaCl, which indicates that the carotenoids production can beenhanced under stress conditions.

Key words: Rhodotorula sp. , Carotenoids , Stress conditions , Response surface analysis

Shi Xinyi, Liu Dan, Chen Lili, Chen Xin. Enhanced Carotenoids Production by a Rhodotorula sp. Under Stress Conditions[J]. Biotechnology Bulletin, 2013, 0(1): 191-197.

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