The Development and Identification of Rift Valley Fever Virus-like Particles

Abstract

Abstract: It was to develop Rift valley fever（RVF）virus-like particles（VLPs）to effectively offer positive material for molecular biological detection. pGEM-T-RVFV plasmid and p-MS2 were individually digested with both Pst I and Hind Ⅲ , and then purified ligated to generate recombinant plasmid p-MS2-RVFV. The plasmid was cotransformed into E. coli. DH5α cell and induced with IPTG, incubated with RNase A and DNase I, and then subsided with salt to obtain the rough-wrought virus-like particles. The method of gradient centrifugation was used to prepared rarefied virus-like particles which was determined for characteristic property. The puried particles were verified by Real-time RT-PCR. Results showed that the rarefied virus-like particles were able to endure RNase A, saved at any rate one month under subzero climatic condition, which indicated that the virus-like particles were stable and were simple transported；it indicated that the VLPs could attain a minimum detectable limit of 4.25×102 copies. The construction of virus-like particles could provide positive material for RVF molecular detection.

Key words: Rift valley fever virus（RVFV）, Virus-like particles（VLPs）, Identification

Deng Junhua, Lin Xiangmei, Zhang Yongning, Wang Caixia, Feng Chunyan, Wu Shaoqiang . The Development and Identification of Rift Valley Fever Virus-like Particles[J]. Biotechnology Bulletin, 2013, 0(2): 135-139.

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