The Production and Application of TRIP-1 Antibody

Biotechnology Bulletin ›› 2013, Vol. 0 ›› Issue (3): 139-143.

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The Production and Application of TRIP-1 Antibody

Zhang Honglin,g Wan Jun, Dong Yichen, Cao Wei, Zhang Xinsheng, Zhang Jinxie, Huang Laiqiang

1. School of Life Sciences,Tsinghua University,Beijing 100084；2. The Shenzhen Key Lab of Gene and Antibody Therapy,Center for Biotech & Biomedicine and Division of Life & Health Sciences,Graduate School at Shenzhen,Tsinghua University,Shenzhen 518055）

Received:2012-08-24 Revised:2013-03-21 Online:2013-03-20 Published:2013-03-21

Abstract

Abstract: To produce antibody against the TGF-β type II receptor binding protein, TRIP-1, contributing to study TRIP-1’s function in TGF-β signaling pathway and cancer development. Human’s TRIP-1 gene was cloned into the prokaryotic expression vector of pET-His, and then transformed into E. coli BL21 to induce TRIP-1 protein expression. Purified TRIP-1 protein was used to immunize New Zealand rabbits and Kunming mice（KM）. The serum was removed and the titer and affinity was determined after immunization 4 times. The antiserum was highly purified by proteinG. Results indicated that the titer of the anti-serum from murine and rabbit are high enough for Western blot and immunofluorescence assay.

Key words: Prokaryotic expression and purification TRIP-1, Animal immunization, Anti-serum, Antibody identification

Zhang Honglin,g Wan Jun, Dong Yichen, Cao Wei, Zhang Xinsheng, Zhang Jinxie, Huang Laiqiang. The Production and Application of TRIP-1 Antibody[J]. Biotechnology Bulletin, 2013, 0(3): 139-143.

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The Production and Application of TRIP-1 Antibody

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