Construction and Analysis of Vip3A Insecticidal Protein Random Recombination Library

Abstract

Abstract: VIPs（Vegetative Insecticidal Proteins）from Bacillus thuringiensis（Bt）are a new class of insecticidal proteins, which enrich the insecticidal spectrum of Bt, and which are important proteins for plant protection. In order to improve the insecticidal activity and expand the insecticidal spectrum of the VIPs, the homologous recombination of vip3Aa39 and vip3Aa11 were completed by Gateway technology, and the products were cloned into the entry vector pDONR221, constructing a mutant library. Result showed 42 recombinant plasmids of different genotypes and 22 mutants of different protein sequences were obtained. The construction and the analysis of the random mutation library makes it possible for getting the Vip proteins with high insecticidal activity, widely insecticidal spectrum and insecticidal specific mechanism research.

Key words: Bacillus thuringiensis, VIPs, Gateway technology, Recombination library

Zhang Ning, Liu Rongmei, Shu Changlong, Zhang Jie, Li Haitao, Gao Jiguo. Construction and Analysis of Vip3A Insecticidal Protein Random Recombination Library[J]. Biotechnology Bulletin, 2013, 0(3): 160-165.

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