Abstract: Based on enrichment culture, initial screening, and further screening by spotting vaccination, a group of degradation lignocelluloses strains were isolated from soil. Then strains were cultured in the flask to develop the repeated screening, and enzymes activity was tested, finally a filamentous fungus 26-6 was screened. Then colonial morphology of the strain on PDA plate was observed, spore morphology was also observed by scanning electron microscope. According to the ITS rDNA sequence analysis, the fungi was initially identified as a Penicillium oxalicum strain and named Penicillium oxalicum EH26-6. Culture optimization was then carried out in flask scale and finally the optimal culture condition was determined as corn cob for carbon source, corn steep powder for organic nitrogen source, pH5.0, temperature 30℃ , inoculation 10%. The research provided an alternative strain for the low cost production of composite cellulose enzyme.

Key words: Isolation, Lignocelluloses, Culture condition, Optimization