Abstract: To compare the transcription activity of original promoter P_GCW14 with other promoters, Candidn antarctic lipase B（CALB） was displayed on the cell surface of Pichia pastoris using P_GCW14, P_AOX1, P_GAP and P_TEF1 as promoter respectively. Anchor protein was a cell wall protein named G_cw14p, which was obtained originally from P. pastoris. In shake-flask cultivation, the activity of P_GCW14 was equal to that of _PAOX1.Under control of P_GCW14 and P_AOX1, the highest lipase hydrolytic activities of recombinant yeasts reached 694.8 U/g（Dry cell weight）and 684.3 U/g（Dry cell weight）after cultivation for 48 h and 120 h, respectively. The fermentation period of recombinant yeast using P_GCW14 as promoter was shortened significantly. While the highest lipase hydrolytic activities of recombinant yeasts controlled by constitutive promoter P_GAP and P_TEF1 reached 266.7 U/g（Dry cell weight）and 449.2 U/g（Dry cell weight）respectively after cultivation for 48 h. This means that the activity of P_GCW14 was higher than that of P_GAP and PTEF1. Furthermore, replacing the α-factor with signal peptide from protein G_cw14p, the lipase hydrolytic activity increased about 4%.

Key words: Pichia pastoris, Promoter, Candidn antarctic lipase B, Yeast cell-surface display